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Signal Transduction Metabolism Energy Metabolism

Methylglyoxal Assay Kit (Fluorometric) (ab273284)

Methylglyoxal Assay Kit (Fluorometric) (ab273284)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Assay type: Quantitative
  • Detection method: Fluorescent
  • Platform: Microplate
  • Sample type: Cell Lysate, Other biological fluids, Tissue Homogenate

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Overview

  • Product name

    Methylglyoxal Assay Kit (Fluorometric)
    See all Methylglyoxal kits
  • Detection method

    Fluorescent
  • Sample type

    Other biological fluids, Cell Lysate, Tissue Homogenate
  • Assay type

    Quantitative
  • Assay duration

    Multiple steps standard assay
  • Product overview

    Methylglyoxal (MG) Assay Activity Kit (Fluorometric) (ab273284) can detect MG using an enzyme-coupled reaction which reduces the fluorogenic probe. The reduced fluorophore produces a stable signal (Ex/Em= 535/587nm), which is directly proportional to the amount of MG in samples.


    The assay is simple, reproducible and can specifically detect as low as 6 pmol of MG in a 100 µl reaction.

  • Platform

    Microplate

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 100 tests
    Enzyme Mix A 1 x 22µl
    Enzyme Mix B 1 x 120µl
    Enzyme Mix C (Lyophilized) 1 vial
    Extraction Solution 1 x 2ml
    MG Assay Buffer 1 x 25ml
    MG Standard (20 mM) 1 x 1.1ml
    Probe (in DMSO) 1 x 0.4ml
    Substrate Mix A (Lyophilized) 1 vial
    Substrate Mix B (Lyophilized) 1 vial
  • Research areas

    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Cardiovascular
    • Atherosclerosis
    • Diabetes associated
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism
  • Relevance

    Methylglyoxal, also called pyruvaldehyde or 2-oxo-propanal (CH3-CO-CH=O or C3H4O2) is the aldehyde form of pyruvic acid. Methylglyoxal is both an aldehyde and a ketone. In the body, methylglyoxal may form from 3-amino acetone, which is an intermediate of threonine catabolism. This compound, as well as glyoxal, is a byproduct of metabolism, but cannot be used. The aldehyde reacts with glutathione, a tripeptide consisting of glutamic acid, cysteine, and glycine. Glutathione reduces methylglyoxal to 1-hydroxy acetone, a ketol. 1-Hydroxy acetone goes through enediol tautomerism to lactaldehyde. Then the lactaldehyde is oxidised by glutathione to D-lactic acid. Methylglyoxal is also called a ketal, because it has an aldehydic and ketonic carbonyl group. It is a type of the advanced glycation endproducts (AGEs) resulting from sugar modifications, such as the Maillard reaction. When the reductone is cleaved, pyruvaldehyde forms.
  • Alternative names

    • 2 oxo propanal
    • MG
    • pyruvaldehyde

Images

  • Methylglyoxal Assay Kit standard curve
    Methylglyoxal Assay Kit standard curve

    Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.

    MG Standard Curve, results from multiple experiments

  • Measurement of MG in Jurkat Cells Lysates
    Measurement of MG in Jurkat Cells Lysates

    Example data.

    MG activity was measured in Jurkat cell ysates (15 µg lysates). All assays were performed following kit protocol.

  • MG activity
    MG activity

    Example data.

    MG activity was measured for mouse liver (4 mg tissue), rat brain (12 mg tissue) and human cerebrospinal fluid (CSF, 2 µl).

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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