Antibodies, Proteins, Kits and Reagents for Life Science

304 886 ₸ Product size

100 µl 304 886 ₸

Order now and get it on Tuesday March 02, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR7366] to PCB
Suitable for: WB
Knockout validated
Reacts with: Mouse, Rat, Human

Product name

Anti-PCB antibody [EPR7366]
See all PCB primary antibodies
Description

Rabbit monoclonal [EPR7366] to PCB
Host species

Rabbit
Tested Applications & Species

Application Species
WB
Mouse

Human

See all applications and species data
Immunogen

Synthetic peptide within Human PCB aa 1050-1150. The exact sequence is proprietary.
Positive control
- Human fetal kidney and liver, HepG2, and A549 lysates; Mouse and Rat brain lysates.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
WB	Mouse Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (K_D)

K_D = 1.15 x 10 ^-10 M
Learn more about K_D
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR7366
Isotype

IgG
Research areas

Western blot - Anti-PCB antibody [EPR7366] (ab126707)

All lanes : Anti-PCB antibody [EPR7366] (ab126707) at 1/1000 dilution

Lane 1 : Wild-type HAP1 whole cell lysate.
Lane 2 : PCB knockout HAP1 whole cell lysate.
Lane 3 : HepG2 whole cell lysate.
Lane 4 : A549 whole cell lysate.

Lysates/proteins at 20 µg per lane.

Predicted band size: 130 kDa

Lanes 1 - 4: Merged signal (red and green). Green - ab126707 observed at 130 kDa. Red - loading control, ab9484, observed at 37 kDa.

ab126707 was shown to recognize PBC in wild-type HAP1 cells as signal was lost at the expected MW in PBC knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and PBC knockout samples were subjected to SDS-PAGE. Ab126707 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-PCB antibody [EPR7366] (ab126707)

All lanes : Anti-PCB antibody [EPR7366] (ab126707) at 1/1000 dilution

Lane 1 : Human fetal kidney lysate
Lane 2 : HepG2 lysate
Lane 3 : Human fetal liver lysate
Lane 4 : A549 lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 130 kDa
Western blot - Anti-PCB antibody [EPR7366] (ab126707)

All lanes : Anti-PCB antibody [EPR7366] (ab126707) at 1/1000 dilution

Lane 1 : Mouse brain lysate
Lane 2 : Rat brain lysate

Predicted band size: 130 kDa
OI-RD Scanning - Anti-PCB antibody [EPR7366] (ab126707)

Equilibrium disassociation constant (K_D)
Learn more about K_D

Click here to learn more about K_D
Anti-PCB antibody [EPR7366] (ab126707)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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