Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PCB antibody [EPR7366] (ab126707)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7366] to PCB
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-PCB antibody [EPR7366]
    See all PCB primary antibodies

  • Description

    Rabbit monoclonal [EPR7366] to PCB

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human PCB aa 1050-1150. The exact sequence is proprietary.

  • Positive control

    • Human fetal kidney and liver, HepG2, and A549 lysates; Mouse and Rat brain lysates.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-PCB antibody [EPR7366] (ab126707)
    Western blot - Anti-PCB antibody [EPR7366] (ab126707)
    All lanes : Anti-PCB antibody [EPR7366] (ab126707) at 1/1000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate.
    Lane 2 : PCB knockout HAP1 whole cell lysate.
    Lane 3 : HepG2 whole cell lysate.
    Lane 4 : A549 whole cell lysate.

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 130 kDa



    Lanes 1 - 4: Merged signal (red and green). Green - ab126707 observed at 130 kDa. Red - loading control, ab9484, observed at 37 kDa.

    ab126707 was shown to recognize PBC in wild-type HAP1 cells as signal was lost at the expected MW in PBC knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and PBC knockout samples were subjected to SDS-PAGE. Ab126707 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-PCB antibody [EPR7366] (ab126707)
    Western blot - Anti-PCB antibody [EPR7366] (ab126707)
    All lanes : Anti-PCB antibody [EPR7366] (ab126707) at 1/1000 dilution

    Lane 1 : Human fetal kidney lysate
    Lane 2 : HepG2 lysate
    Lane 3 : Human fetal liver lysate
    Lane 4 : A549 lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 130 kDa

  • Western blot - Anti-PCB antibody [EPR7366] (ab126707)
    Western blot - Anti-PCB antibody [EPR7366] (ab126707)
    All lanes : Anti-PCB antibody [EPR7366] (ab126707) at 1/1000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Rat brain lysate

    Predicted band size: 130 kDa

  • OI-RD Scanning - Anti-PCB antibody [EPR7366] (ab126707)
    OI-RD Scanning - Anti-PCB antibody [EPR7366] (ab126707)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-PCB antibody [EPR7366] (ab126707)
    Anti-PCB antibody [EPR7366] (ab126707)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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