Anti-PCB antibody - C-terminal (ab229266)
Key features and details
- Rabbit polyclonal to PCB - C-terminal
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
-
Product name
Anti-PCB antibody - C-terminal
See all PCB primary antibodies -
Description
Rabbit polyclonal to PCB - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow, Pig, Rhesus monkey
-
Immunogen
Recombinant fragment within Human PCB (C terminal). The exact sequence is proprietary.
Database link: P11498 -
Positive control
- WB: HEK-293T, A431 and HeLa whole cell lysate; mouse liver tissue lysate. IHC-P: Mouse liver tissue. ICC/IF: HeLa cells.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-PCB antibody - C-terminal (ab229266)All lanes : Anti-PCB antibody - C-terminal (ab229266) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : Mouse liver tissue lysate
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.5% SDS-PAGE
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCB antibody - C-terminal (ab229266)Paraffin-embedded mouse liver tissue stained for PCB using ab229266 at 1/500 dilution in immunohistochemical analysis.
-
Immunocytochemistry/ Immunofluorescence - Anti-PCB antibody - C-terminal (ab229266)HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for PCB using ab229266 (green) at 1/500 dilution in ICC/IF. Cells were fixed in ice-cold methanol for 5 minutes. Blue: Hoechst 33342 staining.
