Anti-PCB antibody [3H2AD9] (ab110314)
Key features and details
- Mouse monoclonal [3H2AD9] to PCB
- Suitable for: ICC/IF, Flow Cyt, IP
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-PCB antibody [3H2AD9]
See all PCB primary antibodies -
Description
Mouse monoclonal [3H2AD9] to PCB -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, Flow Cyt, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length native protein (purified). This information is considered to be commercially sensitive.
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Positive control
- Human fibroblast and HeLa cells; Human liver tissue lysate.
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General notes
This antibody clone is manufactured by Abcam.
Product was previously marketed under the MitoSciences sub-brand.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading...
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Purification notes
ab110314 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. Purity: >95% by SDS-PAGE. -
Clonality
Monoclonal -
Clone number
3H2AD9 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Upper image shows: Immunocytochemistry analysis using ab110314 at 5µg/ml staining PCB in Human fibroblast cells (4% paraformaldehyde fixed and 0.1% Triton X-100 permeabilized) followed by Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour (red).
Note: The target protein locates to the mitochondrial matrix.
Lower image shows cells co-stained with an antibody against PDH (green), an enzyme also located in the mitochondrial matrix. The composite image shows an identical mitochondrial pattern for both antibodies indicated by merged orange color. -
Detection of ab110314 by immunoprecipitation staining of 130kDa PCB in human liver lysate.
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Flow cytometric analysis using ab110314 at 1µg/ml staining PCB in HeLa cells (blue). Isotype control antibody (red).