Antibodies, Proteins, Kits and Reagents for Life Science

Anti-PHD finger protein 6/PHF6 antibody [EPR11996(B)] - BSA and Azide free (ab249600)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR11996(B)] to PHD finger protein 6/PHF6 - BSA and Azide free
Suitable for: ICC/IF, WB
Knockout validated
Reacts with: Human

Product name

Anti-PHD finger protein 6/PHF6 antibody [EPR11996(B)] - BSA and Azide free
See all PHD finger protein 6/PHF6 primary antibodies
Description

Rabbit monoclonal [EPR11996(B)] to PHD finger protein 6/PHF6 - BSA and Azide free
Host species

Rabbit
Tested Applications & Species

Application Species
WB
Human

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HeLa and HEK-293T cell lysates.
General notes
ab249600 is the carrier-free version of ab170929 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab249600 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

Maxpar® is a trademark of Fluidigm Canada Inc.

This product was previously labelled as PHD finger protein 6
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.2
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Purity

Affinity purified
Clonality

Monoclonal
Clone number

EPR11996(B)
Isotype

IgG
Research areas

Western blot - Anti-PHD finger protein 6/PHF6 antibody [EPR11996(B)] - BSA and Azide free (ab249600)

All lanes : Anti-PHD finger protein 6/PHF6 antibody [EPR11996(B)] (ab170929) at 1/1000 dilution

Lane 1 : Wild-type HeLa cell lysate
Lane 2 : PHF6 knockout HeLa cell lysate
Lane 3 : Wild-type HEK-293T cell lysate
Lane 4 : PHF6 knockout HEK-293 cell lysate

Lysates/proteins at 20 µg per lane.

Performed under reducing conditions.

Predicted band size: 41 kDa

This data was developed using the same antibody clone in a different buffer formulation (ab170929).

Lanes 1-4: Merged signal (red and green). Green - ab170929 observed at 43 kDa. Red - loading control ab7291 observed at 50 kDa.

ab170929 Recombinant Anti-PHF6 antibody [EPR11996(B)] was shown to specifically react with PHF6 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264902 (knockout cell lysate ab258110) was used. Wild-type and PHF6 knockout samples were subjected to SDS-PAGE. ab170929 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Anti-PHD finger protein 6/PHF6 antibody [EPR11996(B)] - BSA and Azide free (ab249600)

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