Anti-SARS-CoV-2 nucleocapsid protein antibody [HL448] - BSA and Azide free (ab281297)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [HL448] to SARS-CoV-2 nucleocapsid protein - BSA and Azide free
- Suitable for: ELISA, WB, ICC, IHC-P, Sandwich ELISA
Overview
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Product name
Anti-SARS-CoV-2 nucleocapsid protein antibody [HL448] - BSA and Azide free
See all SARS-CoV-2 nucleocapsid protein primary antibodies -
Description
Rabbit monoclonal [HL448] to SARS-CoV-2 nucleocapsid protein - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: ELISA, WB, ICC, IHC-P, Sandwich ELISAmore details -
Species reactivity
Reacts with: SARS-CoV-2 -
Immunogen
Recombinant full length protein corresponding to Human coronavirus SARS-CoV-2 nucleocapsid protein. Strain Wuhan-Hu-1
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Positive control
- IHC-P: SARS-CoV-2 (COVID-19) nucleocapsid transfected 293T cells. ICC: SARS-CoV-2 (COVID-19) nucleocapsid transfected 293T cells. WB: SARS-CoV-2 (COVID-19) nucleocapsid transfected 293T cells, SARS-CoV-2 (COVID-19) nucleocapsid transfected Vero E6 cells. ELISA: Recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.4
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
HL448 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-SARS-CoV-2 nucleocapsid protein antibody [HL448] - BSA and Azide free (ab281297) at 1/5000 dilution
Lane 1 : 293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extracts
Lane 2 : SARS-CoV NP transfected 293T whole cell extracts
Lane 3 : SARS-CoV-2 (COVID-19) NP transfected 293T whole cell extracts
Lane 4 : MERS-CoV NP transfected 293T whole cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
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Paraffin embedded infected human placenta (left) and normal human placenta (right) tissue stained for SARS-CoV-2 (COVID-19) nucleocapsid protein using ab281297 in immunohistochemical analysis.
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Paraffin embedded infected human lung tissue stained for SARS-CoV-2 (COVID-19) nucleocapsid protein using ab281297 in immunohistochemical analysis.
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Sandwich ELISA detection of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag protein using ab281297 as capture antibody at concentration of 5 µg/mL and HRP-conjugated ab281295 as detection antibody at concentration of 1 µg/mL.
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Mock and SARS-CoV-2 (COVID-19) nucleocapsid transfected 293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes. SARS-CoV-2 (COVID-19) nucleocapsid was stained by ab281297 (green) at 1/1000 dilution by immunofluorescent analysis. Fluoroshield with DAPI (blue).
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Mock and SARS-CoV-2 (COVID-19) nucleocapsid transfected 293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell FFPE Cell Pellet Block. SARS-CoV-2 (COVID-19) nucleocapsid was stained by ab281297 (green) at 1/1000 dilution by immunohistochemical analysis. Fluoroshield with DAPI (blue).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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Indirect ELISA analysis performed by coating plate with recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein (50 ng). Coated protein probed with ab281297 (4.8*10⁻⁶ ~ 20 nM). HRP-conjugated Rabbit IgG antibody at 1/10000 dilution detected bound primary antibody.
EC50: 0.011 nM -
All lanes : Anti-SARS-CoV-2 nucleocapsid protein antibody [HL448] - BSA and Azide free (ab281297) at 1/5000 dilution
Lane 1 : Vero E6 whole cell extracts
Lane 2 : SARS-CoV-2 (COVID-19) nucleocapsid transfected Vero E6 whole cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
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Indirect ELISA analysis performed by coating plate with recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag (1000-15.625 ng/mL). Coated protein was probed with ab281297 (1 μg/mL). HRP-conjugated Rabbit IgG antibody at 1/10000 dilution was used to detect bound primary antibody.
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All lanes : Anti-SARS-CoV-2 nucleocapsid protein antibody [HL448] - BSA and Azide free (ab281297) at 1/5000 dilution
Lane 1 : 293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extracts
Lane 2 : SARS-CoV-2 (COVID-19) NP transfected 293T whole cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody