Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SARS-CoV-2 nucleocapsid protein antibody [HL448] - BSA and Azide free (ab281297)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [HL448] to SARS-CoV-2 nucleocapsid protein - BSA and Azide free
  • Suitable for: ELISA, WB, ICC, IHC-P, Sandwich ELISA

Overview

  • Product name

    Anti-SARS-CoV-2 nucleocapsid protein antibody [HL448] - BSA and Azide free
    See all SARS-CoV-2 nucleocapsid protein primary antibodies

  • Description

    Rabbit monoclonal [HL448] to SARS-CoV-2 nucleocapsid protein - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ELISA, WB, ICC, IHC-P, Sandwich ELISAmore details

  • Species reactivity

    Reacts with: SARS-CoV-2

  • Immunogen

    Recombinant full length protein corresponding to Human coronavirus SARS-CoV-2 nucleocapsid protein. Strain Wuhan-Hu-1

  • Positive control

    • IHC-P: SARS-CoV-2 (COVID-19) nucleocapsid transfected 293T cells. ICC: SARS-CoV-2 (COVID-19) nucleocapsid transfected 293T cells. WB: SARS-CoV-2 (COVID-19) nucleocapsid transfected 293T cells, SARS-CoV-2 (COVID-19) nucleocapsid transfected Vero E6 cells. ELISA: Recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein.

Properties

Images

  • Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL448] - BSA and Azide free (ab281297)
    Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL448] - BSA and Azide free (ab281297)
    All lanes : Anti-SARS-CoV-2 nucleocapsid protein antibody [HL448] - BSA and Azide free (ab281297) at 1/5000 dilution

    Lane 1 : 293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extracts
    Lane 2 : SARS-CoV NP transfected 293T whole cell extracts
    Lane 3 : SARS-CoV-2 (COVID-19) NP transfected 293T whole cell extracts
    Lane 4 : MERS-CoV NP transfected 293T whole cell extracts

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG antibody
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - (ab281297)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - (ab281297)
    Paraffin embedded infected human placenta (left) and normal human placenta (right) tissue stained for SARS-CoV-2 (COVID-19) nucleocapsid protein using ab281297 in immunohistochemical analysis.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - (ab281297)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - (ab281297)
    Paraffin embedded infected human lung tissue stained for SARS-CoV-2 (COVID-19) nucleocapsid protein using ab281297 in immunohistochemical analysis.
  • Sandwich ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL448] - BSA and Azide free (ab281297)
    Sandwich ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL448] - BSA and Azide free (ab281297)

    Sandwich ELISA detection of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag protein using ab281297 as capture antibody at concentration of 5 µg/mL and HRP-conjugated ab281295 as detection antibody at concentration of 1 µg/mL.

  • Immunocytochemistry - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL448] - BSA and Azide free (ab281297)
    Immunocytochemistry - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL448] - BSA and Azide free (ab281297)

     

    Mock and SARS-CoV-2 (COVID-19) nucleocapsid transfected 293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes. SARS-CoV-2 (COVID-19) nucleocapsid was stained by ab281297 (green) at 1/1000 dilution by immunofluorescent analysis. Fluoroshield with DAPI (blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - (ab281297)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - (ab281297)

    Mock and SARS-CoV-2 (COVID-19) nucleocapsid transfected 293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell FFPE Cell Pellet Block. SARS-CoV-2 (COVID-19) nucleocapsid was stained by ab281297 (green) at 1/1000 dilution by immunohistochemical analysis. Fluoroshield with DAPI (blue).

    Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

  • Indirect ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL448] - BSA and Azide free (ab281297)
    Indirect ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL448] - BSA and Azide free (ab281297)

    Indirect ELISA analysis performed by coating plate with recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein (50 ng). Coated protein probed with ab281297 (4.8*10⁻⁶ ~ 20 nM). HRP-conjugated Rabbit IgG antibody at 1/10000 dilution detected bound primary antibody.
    EC50: 0.011 nM

  • Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL448] - BSA and Azide free (ab281297)
    Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL448] - BSA and Azide free (ab281297)
    All lanes : Anti-SARS-CoV-2 nucleocapsid protein antibody [HL448] - BSA and Azide free (ab281297) at 1/5000 dilution

    Lane 1 : Vero E6 whole cell extracts
    Lane 2 : SARS-CoV-2 (COVID-19) nucleocapsid transfected Vero E6 whole cell extracts

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG antibody
  • Indirect ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL448] - BSA and Azide free (ab281297)
    Indirect ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL448] - BSA and Azide free (ab281297)

    Indirect ELISA analysis performed by coating plate with recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag (1000-15.625 ng/mL). Coated protein was probed with ab281297 (1 μg/mL). HRP-conjugated Rabbit IgG antibody at 1/10000 dilution was used to detect bound primary antibody.

  • Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL448] - BSA and Azide free (ab281297)
    Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL448] - BSA and Azide free (ab281297)
    All lanes : Anti-SARS-CoV-2 nucleocapsid protein antibody [HL448] - BSA and Azide free (ab281297) at 1/5000 dilution

    Lane 1 : 293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extracts
    Lane 2 : SARS-CoV-2 (COVID-19) NP transfected 293T whole cell extracts

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG antibody

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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