Anti-SA2 antibody [EPR17865] - BSA and Azide free (ab251341)
![Anti-SA2 antibody [EPR17865] - BSA and Azide free (ab251341)](https://www.abcam.com/ps/products/251/ab251341/Images/ab251341-391469-anti-sa2-antibody-epr17865-c-terminal-western-blot-human-knockout-lysate.jpg "Anti-SA2 antibody [EPR17865] - BSA and Azide free (ab251341)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17865] to SA2 - BSA and Azide free
- Suitable for: WB, Flow Cyt, IHC-P, ICC/IF
- Knockout validated
- Reacts with: Mouse, Rat, Human
Overview
-
Product name
Anti-SA2 antibody [EPR17865] - BSA and Azide free
See all SA2 primary antibodies -
Description
Rabbit monoclonal [EPR17865] to SA2 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cyt, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: HeLa and HCT 116 cell lysates.
-
General notes
Ab251341 is the carrier-free version of ab201451. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab251341 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
EPR17865 -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-SA2 antibody [EPR17865] - BSA and Azide free (ab251341)All lanes : Anti-SA2 antibody [EPR17865] - C-terminal (ab201451) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : STAG2 knockout HeLa cell lysate
Lane 3 : HCT116 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 141 kDa
Observed band size: 141 kDaThis data was developed using the same antibody clone in a different buffer formulation (ab201451).
Lanes 1-3: Merged signal (red and green). Green - ab201451 observed at 141 kDa. Red - loading control ab8245 observed at 36 kDa.
ab201451 Anti-SA2 antibody [EPR17865] - C-terminal was shown to specifically react with SA2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265461 (knockout cell lysate ab257707) was used. Wild-type and SA2 knockout samples were subjected to SDS-PAGE. ab201451 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
-
![Anti-SA2 antibody [EPR17865] - BSA and Azide free (ab251341)](https://www.abcam.com/ps/products/251/ab251341/Images/ab251341-3-benefits-of-recombinant-antibodies.png)
Anti-SA2 antibody [EPR17865] - BSA and Azide free (ab251341)
