Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-RYBP antibody [EPR13059(2)] - BSA and Azide free (ab250871)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13059(2)] to RYBP - BSA and Azide free
  • Suitable for: IP, IHC-P, Flow Cyt, ICC/IF, ChIP, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-RYBP antibody [EPR13059(2)] - BSA and Azide free
    See all RYBP primary antibodies

  • Description

    Rabbit monoclonal [EPR13059(2)] to RYBP - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ChIP
    Human
    Flow Cyt
    Human
    IP
    Mouse
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IP: NIH/3T3 cell lysates Flow Cyt: A431 cells

  • General notes

    Ab250871 is the carrier-free version of ab185971. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab250871 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunoprecipitation - Anti-RYBP antibody [EPR13059(2)] - BSA and Azide free (ab250871)
    Immunoprecipitation - Anti-RYBP antibody [EPR13059(2)] - BSA and Azide free (ab250871)

    ab185971 (purified) at 1/20 dilution(11.7µg/mL) immunoprecipitating RYBP in NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate 10 µg.

    Lane 1 (input): NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate 10 µg
    Lane 2 (+): ab185971 & NIH/3T3 whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab185971 in NIH/3T3 whole cell lysate.

    For western blotting, ab185971 at 1/500 dilution (0.468 µg/mL) and veriBlot for IP secondary antibody (HRP) (ab131366) at 1/1000 dilution was used.

    Blocking and diluting buffer: 5% NFDM /TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185971)

  • Flow Cytometry - Anti-RYBP antibody [EPR13059(2)] - BSA and Azide free (ab250871)
    Flow Cytometry - Anti-RYBP antibody [EPR13059(2)] - BSA and Azide free (ab250871)

    Flow Cytometry analysis of A431 (Human epidermoid carcinoma epithelial cell) cells labeling RYBP with purified ab185971 at 1/20 dilution (11.7 µg/mL) (Red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) (black). Unlabeled control - Unlabelled cells (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185971).

  • ChIP - Anti-RYBP antibody [EPR13059(2)] - BSA and Azide free (ab250871)
    ChIP - Anti-RYBP antibody [EPR13059(2)] - BSA and Azide free (ab250871)

    Chromatin was prepared from NIH/3T3 cells according to the Abcam Dual X-ChIP protocol*. Cells were fixed with EGS for 30 minutes, then formaldehyde for 10 minutes.
    The ChIP was performed with 25 µg of chromatin, 5 µg of ab185971 (red), and 20 µl of Protein A/G sepharose beads. 5 µg of rabbit normal IgG was added to the beads control (gray). The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach).
    Primers and probes are located in the first kb of the transcribed region.
    *http://www.abcam.com/resources?keywords=X%20ChIP%20protocol
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185971).

  • ChIP - Anti-RYBP antibody [EPR13059(2)] - BSA and Azide free (ab250871)
    ChIP - Anti-RYBP antibody [EPR13059(2)] - BSA and Azide free (ab250871)

    Chromatin was prepared from HEK293 cells according to the Abcam Dual X-ChIP protocol*. Cells were fixed with EGS for 30 minutes, then formaldehyde for 10 minutes.
    The ChIP was performed with 25 µg of chromatin, 5 µg of ab185971 (red), and 20 µl of Protein A/G sepharose beads. 5 µg of rabbit normal IgG was added to the beads control (gray). The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach).
    Primers and probes are located in the first kb of the transcribed region.
    *http://www.abcam.com/resources?keywords=X%20ChIP%20protocol
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185971).

  • Anti-RYBP antibody [EPR13059(2)] - BSA and Azide free (ab250871)
    Anti-RYBP antibody [EPR13059(2)] - BSA and Azide free (ab250871)

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