Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CDK1 + CDK2 + CDK3 + CDK5 (phospho Y15) antibody [EPR7875] - BSA and Azide free (ab248508)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7875] to CDK1 + CDK2 + CDK3 + CDK5 (phospho Y15) - BSA and Azide free
  • Suitable for: ELISA, IHC-P, WB, Dot blot
  • Reacts with: Mouse, Rat, Human, Recombinant fragment

Overview

  • Product name

    Anti-CDK1 + CDK2 + CDK3 + CDK5 (phospho Y15) antibody [EPR7875] - BSA and Azide free

  • Description

    Rabbit monoclonal [EPR7875] to CDK1 + CDK2 + CDK3 + CDK5 (phospho Y15) - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    The antibody will cross-react with phosphorylated CDK1 (pY15), CDK2 (pY15), CDK3 (pY15) and CDK5 (pY15) but not with non-phosphorylated CDK1, CDK2, CDK3 and CDK5. Please see our ELISA results on the images section.

  • Tested applications

    Suitable for: ELISA, IHC-P, WB, Dot blotmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human, Recombinant fragment

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab248508 is the carrier-free version of ab133463.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-CDK1 + CDK2 + CDK3 + CDK5 (phospho Y15) antibody [EPR7875] - BSA and Azide free (ab248508)
    Western blot - Anti-CDK1 + CDK2 + CDK3 + CDK5 (phospho Y15) antibody [EPR7875] - BSA and Azide free (ab248508)
    Anti-CDK1 + CDK2 + CDK3 + CDK5 (phospho Y15) antibody [EPR7875] (ab133463) at 1/1000 dilution (purified) + HeLa cell lysate treated with UV at 10 µg

    Secondary
    HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 34 kDa
    Observed band size: 34 kDa



    This data was developed using ab133463, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK1 + CDK2 + CDK3 + CDK5 (phospho Y15) antibody [EPR7875] - BSA and Azide free (ab248508)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK1 + CDK2 + CDK3 + CDK5 (phospho Y15) antibody [EPR7875] - BSA and Azide free (ab248508)

    This data was developed using ab133463, the same antibody clone in a different buffer formulation.

    Immunohistochemical staining of paraffin embedded human colon with purified ab133463 at a working dilution of 1 in 75. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
  • ELISA - Anti-CDK1 + CDK2 + CDK3 + CDK5 (phospho Y15) antibody [EPR7875] - BSA and Azide free (ab248508)
    ELISA - Anti-CDK1 + CDK2 + CDK3 + CDK5 (phospho Y15) antibody [EPR7875] - BSA and Azide free (ab248508)

    This data was developed using ab133463, the same antibody clone in a different buffer formulation.

    Direct ELISA antibody dose-response curve using purified ab133463 at 0-1000 ng/ml. Antigen concentration of 1000 ng/mL. An alkaline phosphatase-conjugated goat anti-rabbit IgG (H+L) (1/2500) was used as the secondary antibody.
  • ELISA - Anti-CDK1 + CDK2 + CDK3 + CDK5 (phospho Y15) antibody [EPR7875] - BSA and Azide free (ab248508)
    ELISA - Anti-CDK1 + CDK2 + CDK3 + CDK5 (phospho Y15) antibody [EPR7875] - BSA and Azide free (ab248508)

    This data was developed using ab133463, the same antibody clone in a different buffer formulation.

    Direct ELISA antibody dose-response curve using purified ab133463 at 0-1000 ng/ml. Antigen concentration of 1000 ng/mL. An alkaline phosphatase-conjugated goat anti-rabbit IgG (H+L) (1/2500) was used as the secondary antibody.
  • Western blot - Anti-CDK1 + CDK2 + CDK3 + CDK5 (phospho Y15) antibody [EPR7875] - BSA and Azide free (ab248508)
    Western blot - Anti-CDK1 + CDK2 + CDK3 + CDK5 (phospho Y15) antibody [EPR7875] - BSA and Azide free (ab248508)
    Anti-CDK1 + CDK2 + CDK3 + CDK5 (phospho Y15) antibody [EPR7875] (ab133463) at 1/2000 dilution (purified) + C6 cell lysate at 10 µg

    Secondary
    HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 34 kDa
    Observed band size: 34 kDa



    This data was developed using ab133463, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-CDK1 + CDK2 + CDK3 + CDK5 (phospho Y15) antibody [EPR7875] - BSA and Azide free (ab248508)
    Western blot - Anti-CDK1 + CDK2 + CDK3 + CDK5 (phospho Y15) antibody [EPR7875] - BSA and Azide free (ab248508)
    Anti-CDK1 + CDK2 + CDK3 + CDK5 (phospho Y15) antibody [EPR7875] (ab133463) at 1/2000 dilution (purified) + NIH/3T3 cell lysate at 10 µg

    Secondary
    HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 34 kDa
    Observed band size: 34 kDa



    This data was developed using ab133463, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Dot Blot - Anti-CDK1 + CDK2 + CDK3 + CDK5 (phospho Y15) antibody [EPR7875] - BSA and Azide free (ab248508)
    Dot Blot - Anti-CDK1 + CDK2 + CDK3 + CDK5 (phospho Y15) antibody [EPR7875] - BSA and Azide free (ab248508)

    This data was developed using ab133463, the same antibody clone in a different buffer formulation.

    Dot blot analysis of CDK1+CDK2+CDK3+CDK5 (pY15) phospho peptide (lane 1) and CDK1+CDK2+CDK3+CDK5 non-phospho peptide (lane 2) labelling CDK1+CDK2+CDK3+CDK5 (phospho Y15) with unpurified ab133463 at a dilution of 1/1000. A peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/2500). Blocking and dilution buffer: 5% NFDM/TBST. Exposure time: 10 seconds.
  • Western blot - Anti-CDK1 + CDK2 + CDK3 + CDK5 (phospho Y15) antibody [EPR7875] - BSA and Azide free (ab248508)
    Western blot - Anti-CDK1 + CDK2 + CDK3 + CDK5 (phospho Y15) antibody [EPR7875] - BSA and Azide free (ab248508)

    This data was developed using ab133463, the same antibody clone in a different buffer formulation.

    Saso2 cells were incubated at 37°C for 24 hours with vehicle control (0 µM) and different concentrations of Amifostine (ab141060). Decreased expression of CDK1 (phospho Y15) (unpurified ab133463) in Saso2 cells correlates with an increase in Amifostine concentration, as described in literature.

    Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 10µg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with unpurified ab133463 at 1 µg/ml and ab32384 at 1 µg/ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10000 and visualised using ECL development solution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK1 + CDK2 + CDK3 + CDK5 (phospho Y15) antibody [EPR7875] - BSA and Azide free (ab248508)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK1 + CDK2 + CDK3 + CDK5 (phospho Y15) antibody [EPR7875] - BSA and Azide free (ab248508)

    This data was developed using ab133463, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin embedded human breast carcinoma tissue labelling CDK1+CDK2+CDK3+CDK5 with unpurified ab133463 at 1/50 dilution. Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Western blot - Anti-CDK1 + CDK2 + CDK3 + CDK5 (phospho Y15) antibody [EPR7875] - BSA and Azide free (ab248508)
    Western blot - Anti-CDK1 + CDK2 + CDK3 + CDK5 (phospho Y15) antibody [EPR7875] - BSA and Azide free (ab248508)
    Anti-CDK1 + CDK2 + CDK3 + CDK5 (phospho Y15) antibody [EPR7875] (ab133463) at 1/1500 dilution (unpurified) + NIH/3T3 cell lysate at 1/1000 dilution

    Secondary
    HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 34 kDa
    Observed band size: 34 kDa



    This data was developed using ab133463, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-CDK1 + CDK2 + CDK3 + CDK5 (phospho Y15) antibody [EPR7875] - BSA and Azide free (ab248508)
    Western blot - Anti-CDK1 + CDK2 + CDK3 + CDK5 (phospho Y15) antibody [EPR7875] - BSA and Azide free (ab248508)
    Anti-CDK1 + CDK2 + CDK3 + CDK5 (phospho Y15) antibody [EPR7875] (ab133463) at 1/1500 dilution (unpurified) + C6 cell lysate at 10 µg

    Secondary
    HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 34 kDa
    Observed band size: 34 kDa



    This data was developed using ab133463, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • OI-RD Scanning - Anti-CDK1 + CDK2 + CDK3 + CDK5 (phospho Y15) antibody [EPR7875] - BSA and Azide free (ab248508)
    OI-RD Scanning - Anti-CDK1 + CDK2 + CDK3 + CDK5 (phospho Y15) antibody [EPR7875] - BSA and Azide free (ab248508)
    This data was developed using ab133463, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Western blot - Anti-CDK1 + CDK2 + CDK3 + CDK5 (phospho Y15) antibody [EPR7875] - BSA and Azide free (ab248508)
    Western blot - Anti-CDK1 + CDK2 + CDK3 + CDK5 (phospho Y15) antibody [EPR7875] - BSA and Azide free (ab248508)
    All lanes : Anti-CDK1 + CDK2 + CDK3 + CDK5 (phospho Y15) antibody [EPR7875] (ab133463) at 1/1000 dilution (unpurified)

    Lane 1 : HeLa cell lysate
    Lane 2 : HeLa cell lysate treated with UV

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit IgG at 1/2000 dilution

    Predicted band size: 34 kDa
    Observed band size: 34 kDa



    This data was developed using ab133463, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK1 + CDK2 + CDK3 + CDK5 (phospho Y15) antibody [EPR7875] - BSA and Azide free (ab248508)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK1 + CDK2 + CDK3 + CDK5 (phospho Y15) antibody [EPR7875] - BSA and Azide free (ab248508)
    This data was developed using ab133463, the same antibody clone in a different buffer formulation.Immunohistochemical staining of paraffin embedded human colon with unpurified ab133463 at a working dilution of 1 in 50. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
  • Anti-CDK1 + CDK2 + CDK3 + CDK5 (phospho Y15) antibody [EPR7875] - BSA and Azide free (ab248508)
    Anti-CDK1 + CDK2 + CDK3 + CDK5 (phospho Y15) antibody [EPR7875] - BSA and Azide free (ab248508)

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