Anti-Cdk1-2-3-5 antibody [EP762RY] (ab206314)
![Anti-Cdk1-2-3-5 antibody [EP762RY] (ab206314)](https://www.abcam.com/ps/products/206/ab206314/Images/ab206314-415230-anti-cdk1-2-3-5-antibody-ep762ry-westernblot-293t-human.jpg "Anti-Cdk1-2-3-5 antibody [EP762RY] (ab206314)")
Key features and details
- Rabbit monoclonal [EP762RY] to Cdk1-2-3-5
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Cdk1-2-3-5 antibody [EP762RY] -
Description
Rabbit monoclonal [EP762RY] to Cdk1-2-3-5 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: human cerebrum tissue WB: 293T transfected with CDK1 expression vector containing a GFP-myc-tag®, whole cell lysate, 293T transfected with CDK2 expression vector containing a GFP-myc-tag®, whole cell lysate, 293T whole cell lysate transfected with CDK3 with GFP-Myc tag and 293T whole cell lysate transfected with CDK5 with GFP-Myc tag
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP762RY -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Cdk1-2-3-5 antibody [EP762RY] (ab206314)All lanes : Anti-Cdk1-2-3-5 antibody [EP762RY] (ab206314) at 1/1000 dilution (Purified)
Lane 1 : 293T (Human embryonic kidney epithelial cell) transfected with an empty vector (vector control) containing a GFP-myc-tag®, whole cell lysate
Lane 2 : 293T (Human embryonic kidney epithelial cell) transfected with CDK1 expression vector containing a GFP-myc-tag®, whole cell lysate
Lane 3 : 293T (Human embryonic kidney epithelial cell) transfected with CDK2 expression vector containing a GFP-myc-tag®, whole cell lysate
Lane 4 : 293T (Human embryonic kidney epithelial cell) whole cell lysate transfected with CDK3 with GFP-Myc tag
Lane 5 : 293T (Human embryonic kidney epithelial cell) whole cell lysate transfected with CDK5 with GFP-Myc tag
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 33 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdk1-2-3-5 antibody [EP762RY] (ab206314)](https://www.abcam.com/ps/products/206/ab206314/Images/ab206314-1-anti-cdk5-immunohistochemistry-cerebrum-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdk1-2-3-5 antibody [EP762RY] (ab206314)Immunohistochemical analysis of paraffin-embedded human cerebrum tissue sections labeling Cdk1-2-3-5 with purified ab206314 at 1/50 dilution. ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Sections were counterstained with Hematoxylin.
Antigen retrieval was heat mediated antigen retrieval using citrate buffer, pH 6.0).