Recombinant human CDK1 + CCNB1 protein (Tagged) (ab104618)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 75% Densitometry
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE, WB
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Product name
Recombinant human CDK1 + CCNB1 protein (Tagged) -
Biological activity
The specific activity of ab104618 was determined to be 14 nmol/min/mg.
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Purity
> 75 % Densitometry.
Purity was determined to be >75% by densitometry. -
Expression system
Baculovirus infected Sf9 cells -
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Tags
GST tag N-Terminus -
Additional sequence information
Tag: N- terminal GST tag on both proteins.
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Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.307% Glutathione, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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Functional Studies - Recombinant human CDK1 + CCNB1 protein (Tagged) (ab104618)The specific activity of CDK1 + CCNB1 (ab104618) was determined to be 5 nmol/min/mg as per activity assay protocol and was equivalent to 16 nmol/min/mg as per radiometric assay
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SDS-PAGE - Recombinant human CDK1 + CCNB1 protein (Tagged) (ab104618)SDS PAGE analysis of ab104618 -
SDS-PAGE - Recombinant human CDK1 + CCNB1 protein (Tagged) (ab104618)SDS PAGE analysis of ab104618 -
Functional Studies - Recombinant human CDK1 + CCNB1 protein (ab104618)Kinase Assay demonstrating specific activity of ab104618. -
SDS-PAGE - Recombinant human CDK1 + CCNB1 protein (ab104618)SDS-PAGE showing ab104618 at approximately 58 and 75 kDa.