Thrombospondin overexpression 293T lysate (whole cell) (ab94095)
Overview
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Product name
Thrombospondin overexpression 293T lysate (whole cell) -
General notes
ab94095 is a 293T cell transfected lysate in which Human Thrombospondin has been transiently over-expressed using a pCMV-Thrombospondin plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
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Tested applications
Suitable for: WBmore details
Properties
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Mycoplasma free
Yes -
Form
Liquid -
Storage instructions
Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles. -
Storage buffer
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Research areas
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Background
Function: Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Similarity: Belongs to the thrombospondin family. Contains 3 EGF-like domains. Contains 1 TSP C-terminal (TSPC) domain. Contains 1 TSP N-terminal (TSPN) domain. Contains 3 TSP type-1 domains. Contains 8 TSP type-3 repeats. Contains 1 VWFC domain.
Images
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SDS-PAGE - Thrombospondin overexpression 293T lysate (whole cell) (ab94095)ab94095 at 15µg/lane on an SDS-PAGE gel.
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Western blot - Thrombospondin overexpression 293T lysate (whole cell) (ab94095)All lanes : Anti-Thrombospondin antibody (ab88529) at 1/500 dilution
Lane 1 : Thrombospondin 293T Transfected Lysate - Positive Control (ab94095)
Lane 2 : 293T Non Transfected Lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
