Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MYH2 antibody [A4.74] (ab264036)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [A4.74] to MYH2
  • Suitable for: IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-MYH2 antibody [A4.74]
    See all MYH2 primary antibodies

  • Description

    Mouse monoclonal [A4.74] to MYH2

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    See all applications and species data

  • Immunogen

    Full length native protein (purified) within Human MYH2. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: Q9UKX2

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • IHC-P: Human skeletal muscle tissue.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYH2 antibody [A4.74] (ab264036)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYH2 antibody [A4.74] (ab264036)

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labeling Myosin (MYH2) with ab264036 at 1/1000 dilution (1.116µg/ml) followed by ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Cytoplasmic staining on human skeletal muscle. The section was incubated with ab264036 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYH2 antibody [A4.74] (ab264036)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYH2 antibody [A4.74] (ab264036)

    Immunohistochemical analysis of paraffin-embedded human liver tissue labeling Myosin (MYH2) with ab264036 at 1/1000 dilution (1.116µg/ml) followed by ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). The section was incubated with ab264036 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Negative control: No staining on human liver (PMID:12899730).  

    Secondary antibody only control: Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Anti-MYH2 antibody [A4.74] (ab264036)
    Anti-MYH2 antibody [A4.74] (ab264036)

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