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Epigenetics and Nuclear Signaling DNA / RNA Translation Ribosome

Anti-RPS6 (phospho S235) antibody (ab131535)

Anti-RPS6 (phospho S235) antibody (ab131535)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to RPS6 (phospho S235)
  • Suitable for: WB, ICC/IF
  • Reacts with: Rat, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-RPS6 (phospho S235) antibody
    See all RPS6 primary antibodies
  • Description

    Rabbit polyclonal to RPS6 (phospho S235)
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Rat
    See all applications and species data
  • Immunogen

    Synthetic phosphopeptide conjugated to KLH, derived from around the phosphorylation site of Serine 235 (

    R-L-S(p)-S-L

    ) of Human RPS6 (NP_001001.2).
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • HeLa cells; PC12 cell extract (anisomycin-treated)

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 0.88% Sodium chloride, 50% Glycerol (glycerin, glycerine), 49% PBS

    PBS without Mg2+ and Ca2+
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab131535 was purified by affinity chromatography using epitope-specific phosphopeptide. Non-phosphospecific antibodies were removed by chromatography using non-phosphopeptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • Translation
    • Ribosome

Images

  • Western blot - Anti-RPS6 (phospho S235) antibody (ab131535)
    Western blot - Anti-RPS6 (phospho S235) antibody (ab131535)
    All lanes : Anti-RPS6 (phospho S235) antibody (ab131535) at 1/500 dilution

    Lane 1 : PC12 cell extract (untreated)
    Lane 2 : PC12 cell extract (anisomycin treated)

    Predicted band size: 29 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-RPS6 (phospho S235) antibody (ab131535)
    Immunocytochemistry/ Immunofluorescence - Anti-RPS6 (phospho S235) antibody (ab131535)
    Immunofluorescence analysis of methanol-fixed HeLa cells labelling RPS6 (phospho S235) with ab131535 at 1/100 dilution.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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