Anti-RPS6 (phospho S235 + S236) antibody (ab264135)
Key features and details
- Rabbit polyclonal to RPS6 (phospho S235 + S236)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RPS6 (phospho S235 + S236) antibody
See all RPS6 primary antibodies -
Description
Rabbit polyclonal to RPS6 (phospho S235 + S236) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide within Human RPS6 (phospho S235 + S236). The exact sequence is proprietary.
Database link: P62753 -
Positive control
- WB: HeLa whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-RPS6 (phospho S235 + S236) antibody (ab264135)All lanes : Anti-RPS6 (phospho S235 + S236) antibody (ab264135) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate, grown in serum containing media
Lane 2 : HeLa whole cell lysate, cultured for 24 hours in serum free
media; At 24h, serum was replaced for 1 h with 50nM Rapamycin
Lane 3 : HeLa whole cell lysate, cultured for 24 hours in serum free media; At 24h, serum was replaced for 1 h without Rapamycin
Lane 4 : HeLa whole cell lysate, cultured for 24 hours in serum free media; after 24h serum starvation was continued with 50nM Rapamycin
Lane 5 : HeLa whole cell lysate, cultured for 24 hours in serum free
media, after 24h serum starvation was continued without Rapamycin
Lysates/proteins at 35 µg per lane.
Predicted band size: 29 kDa
Exposure time: 10 seconds4-20% SDS-PAGE.
The blot was stripped and re-blotted for total RPS6 using an affinity purified rabbit anti-RPS6 antibody at 0.04 μg/ml.
Detection: Chemiluminescence with exposure times of 10 seconds (upper panel) or 30 seconds (lower panel).
