Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-RPL10A antibody [EPR12344] (ab174318)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12344] to RPL10A
  • Suitable for: WB, ICC/IF, Flow Cyt, IHC-P
  • Reacts with: Rat, Human

Overview

  • Product name

    Anti-RPL10A antibody [EPR12344]
    See all RPL10A primary antibodies

  • Description

    Rabbit monoclonal [EPR12344] to RPL10A

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment within Human RPL10A. The exact sequence is proprietary.
    Database link: P62906

  • Positive control

    • HepG2 and PC-12 lysates. Human endometrial adenocarcinoma tissue. Jurkat and HeLa cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR12344

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-RPL10A antibody [EPR12344] (ab174318)
    Western blot - Anti-RPL10A antibody [EPR12344] (ab174318)
    All lanes : Anti-RPL10A antibody [EPR12344] (ab174318) at 1/1000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : PC-12 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-Rabbit at 1/2000 dilution

    Predicted band size: 25 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL10A antibody [EPR12344] (ab174318)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL10A antibody [EPR12344] (ab174318)

    Immunohistochemical analysis of paraffin-embedded Human endometrial adenocarcinoma tissue labelling RPL10A using ab174318 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-RPL10A antibody [EPR12344] (ab174318)
    Immunocytochemistry/ Immunofluorescence - Anti-RPL10A antibody [EPR12344] (ab174318)

    Immunofluorescence analysis of HeLa cells labelling RPL10A with ab174318 at 1/100 dilution (red) and DAPI nuclear staining (blue).

  • Flow Cytometry - Anti-RPL10A antibody [EPR12344] (ab174318)
    Flow Cytometry - Anti-RPL10A antibody [EPR12344] (ab174318)

    Flow cytometric analysis of permeabilized Jurkat cells labeling  RPL10A with ab174318 at 1/10 dilution (red) or a rabbit IgG (negative) (green).

  • Anti-RPL10A antibody [EPR12344] (ab174318)
    Anti-RPL10A antibody [EPR12344] (ab174318)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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