Anti-RPL10A antibody (ab187998)
Key features and details
- Rabbit polyclonal to RPL10A
- Suitable for: WB, IHC-P, ICC
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RPL10A antibody
See all RPL10A primary antibodies -
Description
Rabbit polyclonal to RPL10A -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICCmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Recombinant fragment corresponding to Human RPL10A aa 100-200 (internal sequence).
Database link: P62906 -
Positive control
- IHC-P: Human pancreas, skin, kidney and small intestine tissue; WB: RT-4 and U-251 MG whole cell lysates; ICC: U-2 OS whole cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling RPL10A in the cytoplasm of exocrine glandular cells with ab187998 at a 1/50 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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All lanes : Anti-RPL10A antibody (ab187998) at 0.4 µg/ml
Lane 1 : RT4 (Human urinary bladder cancer cell line) whole cell lysate
Lane 2 : U-251 MG (formally U-373 MG) (Human brain glioma cell line) whole cell lysate
Predicted band size: 25 kDa
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Immunocytochemical analysis of U-2 OS (Human bone osteosarcoma epithelial cell line) whole cells labeling RPL10A in the nucleoli, cytosol and endoplasmic reticulum with ab187998 at 2 µg/ml.
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Immunohistochemical analysis of paraffin-embedded human skin tissue labeling RPL10A in the cytoplasm of squamous epithelial cells with ab187998 at a 1/50 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded human small intestine tissue labeling RPL10A in the cytoplasm of glandular cells with ab187998 at a 1/50 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling RPL10A in the cytoplasm of cells in tubules with ab187998 at a 1/50 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.