Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-RPL11 antibody (ab79352)

Key features and details

  • Rabbit polyclonal to RPL11
  • Suitable for: IHC-P, WB, IP
  • Reacts with: Human, Zebrafish
  • Isotype: IgG

Overview

  • Product name

    Anti-RPL11 antibody
    See all RPL11 primary antibodies

  • Description

    Rabbit polyclonal to RPL11

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WB, IPmore details

  • Species reactivity

    Reacts with: Human, Zebrafish
    Predicted to work with: Mouse, Rat, Cow, Cat, Orangutan

  • Immunogen

    Synthetic peptide corresponding to Human RPL11 aa 150 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab88221)

Properties

Applications

Our Abpromise guarantee covers the use of ab79352 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Detects a band of approximately 20 kDa (predicted molecular weight: 22 kDa).
IP Use at an assay dependent concentration.

Target

  • Function

    Binds to 5S ribosomal RNA (By similarity). Required for rRNA maturation and formation of the 60S ribosomal subunits. Promotes nucleolar location of PML.

  • Involvement in disease

    Defects in RPL11 are the cause of Diamond-Blackfan anemia type 7 (DBA7) [MIM:612562]. DBA7 is a form of Diamond-Blackfan anemia, a congenital non-regenerative hypoplastic anemia that usually presents early in infancy. Diamond-Blackfan anemia is characterized by a moderate to severe macrocytic anemia, erythroblastopenia, and an increased risk of malignancy. 30 to 40% of Diamond-Blackfan anemia patients present with short stature and congenital anomalies, the most frequent being craniofacial (Pierre-Robin syndrome and cleft palate), thumb and urogenital anomalies.

  • Sequence similarities

    Belongs to the ribosomal protein L5P family.

  • Cellular localization

    Nucleus > nucleolus.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • 60S ribosomal protein L11 antibody
    • Cell growth inhibiting protein 34 antibody
    • Cell growth inhibiting protein34 antibody
    • CLL associated antigen KW 12 antibody
    • CLL associated antigen KW12 antibody
    • CLL-associated antigen KW-12 antibody
    • DBA 7 antibody
    • DBA7 antibody
    • GIG 34 antibody
    • GIG34 antibody
    • L11 antibody
    • Ribosomal protein L11 antibody
    • RL11_HUMAN antibody
    • RPL 11 antibody
    • Rpl11 antibody
    see all

Images

  • Western blot - Anti-RPL11 antibody (ab79352)
    Western blot - Anti-RPL11 antibody (ab79352)
    All lanes : Anti-RPL11 antibody (ab79352) at 1 µg/ml

    Lane 1 : Human liver tissue lysate - total protein (ab29889)
    Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 3 : Human brain tissue lysate - total protein (ab29466)
    Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 5 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 6 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
    Lane 7 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 22 kDa
    Observed band size: 22 kDa
    Additional bands at: 30 kDa, 45 kDa, 70 kDa. We are unsure as to the identity of these extra bands.



    RPL11 contains a number of potential phosphorylation and acetylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
  • Immunoprecipitation - Anti-RPL11 antibody (ab79352)
    Immunoprecipitation - Anti-RPL11 antibody (ab79352)
    RPL11 was immunoprecipitated using 0.5mg Jurkat whole cell extract, 5µg of Rabbit polyclonal to RPL11 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Jurkat whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab79352.
    Secondary: Clean blot (HRP conjugate) at 1/1000 dilution.
    Band: 22kDa: RPL11
  • - Anti-RPL11 antibody (ab79352)
    - Anti-RPL11 antibody (ab79352)
    IHC image of ab79352 staining in human pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab79352, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  • Western blot - Anti-RPL11 antibody (ab79352)
    Western blot - Anti-RPL11 antibody (ab79352)
    All lanes : Anti-RPL11 antibody (ab79352) at 1 µg/ml

    Lane 1 : Marker
    Lane 2 : Zebrafish brain homogenate at 20 µg
    Lane 3 : Zebrafish heart homogenate at 20 µg
    Lane 4 : Zebrafish liver homogenate at 20 µg
    Lane 5 : Zebrafish skeletal muscle homogenate at 20 µg
    Lane 6 : Human hepatocellular carcinoma cell line at 20 µg

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 22 kDa
    Observed band size: 22 kDa


    Exposure time: 3 minutes

References (10)

Publishing research using ab79352? Please let us know so that we can cite the reference in this datasheet.

ab79352 has been referenced in 10 publications.

  • Pinho M  et al. NOL12 Repression Induces Nucleolar Stress-Driven Cellular Senescence and Is Associated with Normative Aging. Mol Cell Biol 39:N/A (2019). PubMed: 30988155
  • Martins T  et al. TGFß/Activin signalling is required for ribosome biogenesis and cell growth in Drosophila salivary glands. Open Biol 7:N/A (2017). PubMed: 28123053
  • Paolini NA  et al. A Ribosomopathy Reveals Decoding Defective Ribosomes Driving Human Dysmorphism. Am J Hum Genet 100:506-522 (2017). PubMed: 28257692
  • O'Brien R  et al. MYC-Driven Neuroblastomas Are Addicted to a Telomerase-Independent Function of Dyskerin. Cancer Res 76:3604-17 (2016). PubMed: 27197171
  • Vartanian S  et al. Application of Mass Spectrometry Profiling to Establish Brusatol as an Inhibitor of Global Protein Synthesis. Mol Cell Proteomics 15:1220-31 (2016). PubMed: 26711467
  • Wang X  et al. Down-regulation of 5S rRNA by miR-150 and miR-383 enhances c-Myc-rpL11 interaction and inhibits proliferation of esophageal squamous carcinoma cells. FEBS Lett 589:3989-97 (2015). WB . PubMed: 26606907
  • Yoon JC  et al. GLTSCR2/PICT1 links mitochondrial stress and Myc signaling. Proc Natl Acad Sci U S A 111:3781-6 (2014). PubMed: 24556985
  • Havel JJ  et al. Nuclear PRAS40 couples the Akt/mTORC1 signaling axis to the RPL11-HDM2-p53 nucleolar stress response pathway. Oncogene N/A:N/A (2014). PubMed: 24704832
  • Danilova N  et al. Ribosomal protein L11 mutation in zebrafish leads to haematopoietic and metabolic defects. Br J Haematol 152:217-28 (2011). PubMed: 21114664
  • Boisvert FM & Lamond AI p53-Dependent subcellular proteome localization following DNA damage. Proteomics : (2010). WB, ICC/IF ; Human . PubMed: 21046615

Images

  • Western blot - Anti-RPL11 antibody (ab79352)
    Western blot - Anti-RPL11 antibody (ab79352)
    All lanes : Anti-RPL11 antibody (ab79352) at 1 µg/ml

    Lane 1 : Human liver tissue lysate - total protein (ab29889)
    Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 3 : Human brain tissue lysate - total protein (ab29466)
    Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 5 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 6 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
    Lane 7 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 22 kDa
    Observed band size: 22 kDa
    Additional bands at: 30 kDa, 45 kDa, 70 kDa. We are unsure as to the identity of these extra bands.



    RPL11 contains a number of potential phosphorylation and acetylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
  • Immunoprecipitation - Anti-RPL11 antibody (ab79352)
    Immunoprecipitation - Anti-RPL11 antibody (ab79352)
    RPL11 was immunoprecipitated using 0.5mg Jurkat whole cell extract, 5µg of Rabbit polyclonal to RPL11 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Jurkat whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab79352.
    Secondary: Clean blot (HRP conjugate) at 1/1000 dilution.
    Band: 22kDa: RPL11
  • - Anti-RPL11 antibody (ab79352)
    - Anti-RPL11 antibody (ab79352)
    IHC image of ab79352 staining in human pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab79352, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  • Western blot - Anti-RPL11 antibody (ab79352)
    Western blot - Anti-RPL11 antibody (ab79352)
    All lanes : Anti-RPL11 antibody (ab79352) at 1 µg/ml

    Lane 1 : Marker
    Lane 2 : Zebrafish brain homogenate at 20 µg
    Lane 3 : Zebrafish heart homogenate at 20 µg
    Lane 4 : Zebrafish liver homogenate at 20 µg
    Lane 5 : Zebrafish skeletal muscle homogenate at 20 µg
    Lane 6 : Human hepatocellular carcinoma cell line at 20 µg

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 22 kDa
    Observed band size: 22 kDa


    Exposure time: 3 minutes

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