Anti-P2X7 antibody [EPR24130-77] (ab259942)
![Anti-P2X7 antibody [EPR24130-77] (ab259942)](https://www.abcam.com/ps/products/259/ab259942/Images/ab259942-451596-anti-p2x7-antibody-epr2413077-western-blot-murine.jpg "Anti-P2X7 antibody [EPR24130-77] (ab259942)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR24130-77] to P2X7
- Suitable for: WB, IHC-P, IP
- Reacts with: Mouse
Overview
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Product name
Anti-P2X7 antibody [EPR24130-77]
See all P2X7 primary antibodies -
Description
Rabbit monoclonal [EPR24130-77] to P2X7 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P MouseIP MouseWB Mouse
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: RAW264.7 whole cell lysate; Mouse brain and lung tissue lysate. IHC-P: Mouse cerebrum, spleen and liver tissue sections. IP: Mouse lung tissue lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR24130-77 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-P2X7 antibody (ab259942)All lanes : Anti-P2X7 antibody [EPR24130-77] (ab259942) at 1/1000 dilution
Lane 1 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate
Lane 2 : Mouse brain tissue lysate
Lane 3 : Mouse lung tissue lysate
Lane 4 : Mouse skeletal muscle tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/100000 dilution
Predicted band size: 69 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
Samples are non-boiled as boiling may cause protein aggregates.
The molecular weight observed is consistent with what has been described in the literature (PMID: 20450227)
The expression profile is consistent with what has been described in the literature (PMID: 29018292)
Low expression or negative control: Mouse skeletal muscle (PMID:15254086, PMID:22415881), which is consistent to IHC expression pattern.
Exposure time: 59 seconds
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - (ab259942)Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling P2X7 with ab259942 at 1/2000 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining in mouse cerebrum (PMID: 28716092).The section was incubated with ab259942 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - (ab259942)Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling P2X7 with ab259942 at 1/2000 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Membranous staining in mouse spleen (PMID: 22415881).The section was incubated with ab259942 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - (ab259942)Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling P2X7 with ab259942 at 1/2000 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Membranous staining in sinusiod of mouse liver (PMID: 22415881).The section was incubated with ab259942 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - (ab259942)Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle tissue labeling P2X7 with ab259942 at 1/2000 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Very weak staining in mouse skeletal muscle.The section was incubated with ab259942 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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Immunoprecipitation - (ab259942)P2X7 was immunoprecipitated from 0.35 mg Mouse lung tissue lysate 10 ug with ab259942 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab259942 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Mouse lung tissue lysate 10 ug
Lane 2: ab259942 IP in Mouse lung tissue lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab259942 in Mouse lung tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3.25 seconds