anti-Rituximab ELISA Kit (Mabthera®) (ab237654)
Key features and details
- Sensitivity: 30 ng/ml
- Range: 62 ng/ml - 500 ng/ml
- Sample type: Plasma, Serum
- Detection method: Colorimetric
- Assay type: Quantitative
- Reacts with: Human
Overview
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Product name
anti-Rituximab ELISA Kit (Mabthera®)
See all Rituximab kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Serum, Plasma -
Assay type
Quantitative -
Sensitivity
30 ng/ml -
Range
62 ng/ml - 500 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 85% - 115% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
anti-Rituximab ELISA Kit (Mabthera®) (ab237654) is designed to quantify/measure the antibody against Rituximab with high specificity and sensitivity in biological matrices.
Rituximab (Mabthera®) is a genetically engineered chimeric murine/human monoclonal antibody specific to CD20. CD20 is an approximately 35 KDa transmembrane phosphoprotein involved in the activation, proliferation, and differentiation of B-lymphocytes. It is absent in terminally differentiated plasma cells. The Fab domain of rituximab binds to the CD20 antigen on B-lymphocytes and the Fc domain recruits immune effector functions to induce apoptosis in B cells. It is used in treating leukemias and lymphomas, some autoimmune disorders, and organ transplant. However, some patients develop unwanted immunogenicity, which leads to production of anti-drug-antibodies (ADAs) inactivating the therapeutic effects of the treatment and, in rare cases, inducing adverse effects.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 96 tests Assay Buffer 1 x 50ml Confirmation Reagent 1 x 12ml Micro ELISA Plate 1 unit Peroxidase Conjugate 1 x 12ml Plate sealers 2 units Rituximab Standard S1 1 x 1ml Rituximab Standard S2 1 x 1ml Rituximab Standard S3 1 x 1ml Rituximab Standard S4 1 x 1ml Rituximab Standard S5 1 x 1ml Rituximab Standard S6 1 x 1ml Rituximab Standard S7 1 x 1ml Stop Solution 1 x 12ml TMB substrate 1 x 12ml Wash buffer (20X) 1 x 50ml -
Research areas
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Relevance
Rituximab is a genetically engineered chimeric murine/human monoclonal antibody specific to CD20. CD20 is an approximately 35 KDa transmembrane phosphoprotein involved in the activation, proliferation, and differentiation of B-lymphocytes. It is absent in terminally differentiated plasma cells. The Fab domain of Rituximab binds to the CD20 antigen on B-lymphocytes and the Fc domain recruits immune effector functions to induce apoptosis in B cells and is used in the treatment of leukemia s and lymphomas, some autoimmune disorders, and organ transplant. -
Cellular localization
Membrane; Multipass membrane protein. -
Alternative names
- B lymphocyte antigen CD20
- B lymphocyte surface antigen B1
- Bp35
see all -
Database links
- Entrez Gene: 931 Human
- Omim: 112210 Human
- SwissProt: P11836 Human
Images
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Standard Curve
Typical Standard Curve: This standard curve is for demonstration only. A standard curve must be run with each assay.
