Rituximab ELISA Kit (Mabthera®) (ab237640)
Key features and details
- Sensitivity: 3 ng/ml
- Range: 3 ng/ml - 300 ng/ml
- Sample type: Plasma, Serum
- Detection method: Colorimetric
- Assay type: Quantitative
- Reacts with: Human
Overview
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Product name
Rituximab ELISA Kit (Mabthera®)
See all Rituximab kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Serum, Plasma -
Assay type
Quantitative -
Sensitivity
3 ng/ml -
Range
3 ng/ml - 300 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 85% - 115% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Rituximab ELISA Kit (Mabthera®) (ab237640) is a highly specific and sensitive kit designed for the in vitro determination of Rituximab in biological matrices such as human serum and plasma. The density of color is proportional to the amount of Rituximab captured from the samples and can be quantified when compared with standard curve.
Rituximab (Mabthera®) is a genetically engineered chimeric murine/human monoclonal antibody specific to CD20. CD20 is an approximately 35 KDa transmembrane phosphoprotein involved in the activation, proliferation and differentiation of B-lymphocytes. It is absent in terminally differentiated plasma cells. The Fab domain of Rituximab binds to the CD20 antigen on B-lymphocytes and the Fc domain recruits immune effector functions to induce apoptosis in B cells and is used in the treatment of leukemias and lymphomas, some autoimmune disorders and organ transplant.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 96 tests Assay Buffer 1 x 50ml HRP-conjugate Probe 1 x 12ml Micro ELISA Plate 1 unit Plate sealers 2 units Rituximab Standard S1 1 x 1ml Rituximab Standard S2 1 x 1ml Rituximab Standard S3 1 x 1ml Rituximab Standard S4 1 x 1ml Rituximab Standard S5 1 x 1ml Rituximab Standard S6 1 x 1ml Rituximab Standard S7 1 x 1ml Rituximab Standard S8 1 x 1ml Stop Solution 1 x 12ml TMB substrate 1 x 12ml Wash buffer (20X) 1 x 50ml -
Research areas
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Relevance
Rituximab is a genetically engineered chimeric murine/human monoclonal antibody specific to CD20. CD20 is an approximately 35 KDa transmembrane phosphoprotein involved in the activation, proliferation, and differentiation of B-lymphocytes. It is absent in terminally differentiated plasma cells. The Fab domain of Rituximab binds to the CD20 antigen on B-lymphocytes and the Fc domain recruits immune effector functions to induce apoptosis in B cells and is used in the treatment of leukemia s and lymphomas, some autoimmune disorders, and organ transplant. -
Cellular localization
Membrane; Multipass membrane protein. -
Alternative names
- B lymphocyte antigen CD20
- B lymphocyte surface antigen B1
- Bp35
see all -
Database links
- Entrez Gene: 931 Human
- Omim: 112210 Human
- SwissProt: P11836 Human
Images
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Example of Rituximab standard curve
Typical Standard Curve: This standard curve is for demonstration only. A standard curve must be run with each assay.
