Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] - BSA and Azide free (ab249097)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9950] to Rho guanine exchange factor 16/NBR - BSA and Azide free
  • Suitable for: ICC/IF, IHC-P, Flow Cyt, WB
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] - BSA and Azide free
    See all Rho guanine exchange factor 16/NBR primary antibodies

  • Description

    Rabbit monoclonal [EPR9950] to Rho guanine exchange factor 16/NBR - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, IHC-P, Flow Cyt, WBmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab249097 is the carrier-free version of ab154785 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab249097 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information. This product was previously labelled as Rho guanine exchange factor 16

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] - BSA and Azide free (ab249097)
    Western blot - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] - BSA and Azide free (ab249097)
    All lanes : Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (ab154785) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : MCF-7 cell lysate
    Lane 3 : 293T cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 80 kDa



    This data was developed using ab154785, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] - BSA and Azide free (ab249097)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] - BSA and Azide free (ab249097)

    This data was developed using ab154785, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human cervix carcinoma tissue labeling Rho guanine exchange factor 16/NBR with ab154785 at 1/50 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] - BSA and Azide free (ab249097)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] - BSA and Azide free (ab249097)

    This data was developed using ab154785, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human normal breast tissue labeling Rho guanine exchange factor 16/NBR with ab154785 at 1/50 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunocytochemistry - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] - BSA and Azide free (ab249097)
    Immunocytochemistry - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] - BSA and Azide free (ab249097)

    This data was developed using ab154785, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of HeLa cells labeling Rho guanine exchange factor 16/NBR with ab154785 at 1/100 dilution.
  • Flow Cytometry - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] - BSA and Azide free (ab249097)
    Flow Cytometry - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] - BSA and Azide free (ab249097)
    This data was developed using ab154785, the same antibody clone in a different buffer formulation.Flow cytometric analysis of permeabilized HeLa cells labeling Rho guanine exchange factor 16/NBR with ab154785 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] - BSA and Azide free (ab249097)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] - BSA and Azide free (ab249097)
    This data was developed using ab154785, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human normal liver tissue using ab154785 showing +ve staining.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] - BSA and Azide free (ab249097)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] - BSA and Azide free (ab249097)
    This data was developed using ab154785, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human skeletal muscle tissue using ab154785 showing +ve staining.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] - BSA and Azide free (ab249097)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] - BSA and Azide free (ab249097)
    This data was developed using ab154785, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human thyroid gland carcinoma tissue using ab154785 showing +ve staining.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] - BSA and Azide free (ab249097)
    Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] - BSA and Azide free (ab249097)

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