Anti-Reptin/TIP49B/RUVB2 antibody (ab36569)
Key features and details
- Rabbit polyclonal to Reptin/TIP49B/RUVB2
- Suitable for: WB, ICC/IF
- Reacts with: Human, Zebrafish
- Isotype: IgG
Overview
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Product name
Anti-Reptin/TIP49B/RUVB2 antibody
See all Reptin/TIP49B/RUVB2 primary antibodies -
Description
Rabbit polyclonal to Reptin/TIP49B/RUVB2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human, Zebrafish
Predicted to work with: Mouse, Rat, Cow, Xenopus laevis
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Immunogen
Synthetic peptide corresponding to Human Reptin/TIP49B/RUVB2 aa 300-400 conjugated to keyhole limpet haemocyanin.
(Peptide available asab36568)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab36569 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 50 kDa). ICC/IF Use a concentration of 1 µg/ml. Target
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Function
Possesses single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (5' to 3') activity. Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. RUVBL2 plays an essential role in oncogenic transformation by MYC and also modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex. May also inhibit the transcriptional activity of ATF2. -
Tissue specificity
Ubiquitously expressed. Highly expressed in testis and thymus. -
Sequence similarities
Belongs to the ruvB family. -
Domain
The C-terminal domain is required for association with ATF2. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus matrix. Nucleus > nucleoplasm. Cytoplasm. Membrane. Mainly localized in the nucleus, associated with nuclear matrix or in the nuclear cytosol. Although it is also present in the cytoplasm and associated with the cell membranes. - Information by UniProt
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Database links
- Entrez Gene: 511048 Cow
- Entrez Gene: 10856 Human
- Entrez Gene: 20174 Mouse
- Entrez Gene: 292907 Rat
- Entrez Gene: 380092 Xenopus laevis
- Entrez Gene: 317678 Zebrafish
- Omim: 604788 Human
- SwissProt: Q2TBU9 Cow
see all -
Alternative names
- 48 kDa TATA box-binding protein-interacting protein antibody
- 48 kDa TBP-interacting protein antibody
- 48-kDa TATA box-binding protein-interacting protein antibody
see all
Images
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Western blot - Anti-Reptin/TIP49B/RUVB2 antibody (ab36569)All lanes : Anti-Reptin/TIP49B/RUVB2 antibody (ab36569) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 :Jurkat whole cell lysate (ab7899)
Lane 3 :A-431 whole cell lysate (ab7909)
Lane 4 :HEK-293 whole cell lysate (ab7902)
Lane 5 : Hep G2 whole cell lysate (ab7900)
Lane 6 : MCF-7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 8 : U2OS Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size: 50 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted? -
Immunocytochemistry/ Immunofluorescence - Anti-Reptin/TIP49B/RUVB2 antibody (ab36569)ICC/IF image of ab36569 stained human HeLa cells. The cells were PFA fixed (10 min) and incubated with the antibody (ab36569, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used to quench autofluorescence. 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red). -
Western blot - Anti-Reptin/TIP49B/RUVB2 antibody (ab36569)This image is courtesy of an anonymous AbreviewAll lanes : Anti-Reptin/TIP49B/RUVB2 antibody (ab36569) at 1 µg/ml
Lane 1 : Hela cells treated with reptin-specific siRNA#1, whole cell lysate
Lane 2 : Hela cells treated with reptin-specific siRNA#2, whole cell lysate
Lane 3 : Hela cells treated with non-specific siRNA, whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Alexa Fluor® 680 conjugated goat anti-rabbit
at 1/7000 dilution
Performed under reducing conditions.
Predicted band size: 50 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Detection method was Odyssey, with 10 second exposure. -
Western blot - Anti-Reptin/TIP49B/RUVB2 antibody (ab36569)All lanes : Anti-Reptin/TIP49B/RUVB2 antibody (ab36569) at 1 µg/ml
Lane 1 : Marker
Lane 2 : Zebrafish brain homogenate at 20 µg
Lane 3 : Zebrafish heart homogenate at 20 µg
Secondary
All lanes : Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 50 kDa
Observed band size: 51 kDa why is the actual band size different from the predicted?
Exposure time: 5 minutes
Protocols
Datasheets and documents
References (15)
ab36569 has been referenced in 15 publications.
- Da Ros M et al. FYCO1 and autophagy control the integrity of the haploid male germ cell-specific RNP granules. Autophagy 13:302-321 (2017). PubMed: 27929729
- Cloutier P et al. R2TP/Prefoldin-like component RUVBL1/RUVBL2 directly interacts with ZNHIT2 to regulate assembly of U5 small nuclear ribonucleoprotein. Nat Commun 8:15615 (2017). WB . PubMed: 28561026
- Milone MR et al. Proteomic characterization of peroxisome proliferator-activated receptor-? (PPAR?) overexpressing or silenced colorectal cancer cells unveils a novel protein network associated with an aggressive phenotype. Mol Oncol 10:1344-62 (2016). PubMed: 27499265
- Healy AR et al. Discovery of a novel ligand that modulates the protein-protein interactions of the AAA+ superfamily oncoprotein reptin. Chem Sci 6:3109-3116 (2015). PubMed: 28706685
- Qiu H et al. Reptin physically interacts with p65 and represses NF-?B activation. FEBS Lett 589:1951-7 (2015). PubMed: 25957047
Images
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Western blot - Anti-Reptin/TIP49B/RUVB2 antibody (ab36569)All lanes : Anti-Reptin/TIP49B/RUVB2 antibody (ab36569) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 :Jurkat whole cell lysate (ab7899)
Lane 3 :A-431 whole cell lysate (ab7909)
Lane 4 :HEK-293 whole cell lysate (ab7902)
Lane 5 : Hep G2 whole cell lysate (ab7900)
Lane 6 : MCF-7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 8 : U2OS Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size: 50 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted? -
Immunocytochemistry/ Immunofluorescence - Anti-Reptin/TIP49B/RUVB2 antibody (ab36569)ICC/IF image of ab36569 stained human HeLa cells. The cells were PFA fixed (10 min) and incubated with the antibody (ab36569, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used to quench autofluorescence. 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).
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Western blot - Anti-Reptin/TIP49B/RUVB2 antibody (ab36569) This image is courtesy of an anonymous AbreviewAll lanes : Anti-Reptin/TIP49B/RUVB2 antibody (ab36569) at 1 µg/ml
Lane 1 : Hela cells treated with reptin-specific siRNA#1, whole cell lysate
Lane 2 : Hela cells treated with reptin-specific siRNA#2, whole cell lysate
Lane 3 : Hela cells treated with non-specific siRNA, whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Alexa Fluor® 680 conjugated goat anti-rabbit
at 1/7000 dilution
Performed under reducing conditions.
Predicted band size: 50 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Detection method was Odyssey, with 10 second exposure. -
Western blot - Anti-Reptin/TIP49B/RUVB2 antibody (ab36569)All lanes : Anti-Reptin/TIP49B/RUVB2 antibody (ab36569) at 1 µg/ml
Lane 1 : Marker
Lane 2 : Zebrafish brain homogenate at 20 µg
Lane 3 : Zebrafish heart homogenate at 20 µg
Secondary
All lanes : Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 50 kDa
Observed band size: 51 kDa why is the actual band size different from the predicted?
Exposure time: 5 minutes
