Anti-CD272/BTLA antibody [FLO67B] - BSA and Azide free (ab252799)
![Anti-CD272/BTLA antibody [FLO67B] - BSA and Azide free (ab252799)](https://www.abcam.com/ps/products/252/ab252799/Images/ab252799-383288-Mouse-monoclonal-CD272-FLO67B-immunohistochemistry-tonsil-human.JPG "Anti-CD272/BTLA antibody [FLO67B] - BSA and Azide free (ab252799)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [FLO67B] to CD272/BTLA - BSA and Azide free
- Suitable for: IHC-P
- Reacts with: Human
Overview
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Product name
Anti-CD272/BTLA antibody [FLO67B] - BSA and Azide free
See all CD272/BTLA primary antibodies -
Description
Mouse monoclonal [FLO67B] to CD272/BTLA - BSA and Azide free -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P Human
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Immunogen
Recombinant fragment (His-tag) within Human CD272/BTLA aa 1-300. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q7Z6A9 -
Positive control
- IHC-P: Human tonsil tissue.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
ab252799 is the carrier-free version of ab241551. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
FLO67B -
Isotype
IgG1 -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD272/BTLA antibody [FLO67B] - BSA and Azide free (ab252799)
Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CD272/BTLA with ab241551 at 0.865µg/ml, followed by ready to use secondary. Positive staining on human tonsil tissue is observed. The section was incubated with ab241551 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use secondary. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab241551).
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![Anti-CD272/BTLA antibody [FLO67B] - BSA and Azide free (ab252799)](https://www.abcam.com/ps/products/252/ab252799/Images/ab252799-2-benefits-of-recombinant-antibodies.png)
Anti-CD272/BTLA antibody [FLO67B] - BSA and Azide free (ab252799)