Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Rb (phospho T821) antibody [E231] - BSA and Azide free (ab208694)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [E231] to Rb (phospho T821) - BSA and Azide free
  • Suitable for: IP, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-Rb (phospho T821) antibody [E231] - BSA and Azide free
    See all Rb primary antibodies

  • Description

    Rabbit monoclonal [E231] to Rb (phospho T821) - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IP: Jurkat whole cell lysate.

  • General notes

    ab208694 is the carrier-free version of ab32015 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab208694 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Rb (phospho T821) antibody [E231] - BSA and Azide free (ab208694)
    Western blot - Anti-Rb (phospho T821) antibody [E231] - BSA and Azide free (ab208694)
    All lanes : Anti-Rb (phospho T821) antibody [E231] (ab32015) at 1/1000 dilution

    Lane 1 : Jurkat cell lysate, untreated
    Lane 2 : Jurkat cell lysate treated with Phosphatase

    Predicted band size: 106 kDa
    Observed band size: 110 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab32015, the same antibody clone in a different buffer formulation.

  • Immunoprecipitation - Anti-Rb (phospho T821) antibody [E231] - BSA and Azide free (ab208694)
    Immunoprecipitation - Anti-Rb (phospho T821) antibody [E231] - BSA and Azide free (ab208694)

    This data was developed using ab32015, the same antibody clone in a different buffer formulation.
    Purified ab32015 at 1/50 dilution (2µg) immunoprecipitating Rb in Jurkat whole cell lysate.
    Lane 1 (input): Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate 10µg
    Lane 2 (+): ab32015 + Jurkat whole cell lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab32015 in Jurkat whole cell lysate.
    VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 110 kDa

  • Anti-Rb (phospho T821) antibody [E231] - BSA and Azide free (ab208694)
    Anti-Rb (phospho T821) antibody [E231] - BSA and Azide free (ab208694)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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