Anti-Rac1 antibody (ab155938)
Key features and details
- Rabbit polyclonal to Rac1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
-
Product name
Anti-Rac1 antibody
See all Rac1 primary antibodies -
Description
Rabbit polyclonal to Rac1 -
Host species
Rabbit -
Specificity
There is high alignment of the immunogen to RAC2 and RAC3 proteins and so it is possible the antibody will also detect RAC2 and RAC3 as well as RAC1. Although any cross reactivity has not been specifically tested in the laboratory. The sizes of these proteins are approximately the same. -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant full length protein corresponding to Human Rac1.
Database link: P63000 -
Positive control
- WB: HeLa, A431, Jurkat, 3T3L1 and 293T cell lysate. IF: Hela cells. IHC: Human breast, prostate and tonsil tissues.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 30% Glycerol, 69% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rac1 antibody (ab155938)Immunohistochemistry analysis of Human breast tissue labeling Rac1 with ab155938 at 1/200.
-
Western blot - Anti-Rac1 antibody (ab155938)All lanes : Anti-Rac1 antibody (ab155938) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : A431 cell lysate
Lane 4 : Jurkat cell lysate
Lane 5 : 3T3L1 cell lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : Goat anti-rabbit-HRP secondary at 1/15000 dilution
Predicted band size: 21 kDa
4-20% Tris-HCl polyacrylamide gel. -
Immunocytochemistry/ Immunofluorescence - Anti-Rac1 antibody (ab155938)Immunofluorescent analysis of Formalin-fixed permeabilized HeLa cells labeling Rac1 with ab155938 at 1/100 in green, F-actin was stain with flurescent phalloidin in red and the nucleus with DAPI in blue. -
Immunocytochemistry/ Immunofluorescence - Anti-Rac1 antibody (ab155938)Immunofluorescent analysis of Formalin-fixed permeabilized Human cells labeling Rac1 with ab155938 at 1/20 in green, F-actin was stain with flurescent phalloidin in red and the nucleus with DAPI in blue. -
Immunocytochemistry/ Immunofluorescence - Anti-Rac1 antibody (ab155938)Immunofluorescent analysis of Formalin-fixed permeabilized Murine cells labeling Rac1 with ab155938 at 1/20 in green, F-actin was stain with flurescent phalloidin in red and the nucleus with DAPI in blue. -
Immunocytochemistry/ Immunofluorescence - Anti-Rac1 antibody (ab155938)Immunofluorescent analysis of Formalin-fixed untreated HeLa cells labeling Rac1 with ab155938 at 1/100 in green, and the nucleus with Hoechst in blue. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rac1 antibody (ab155938)Immunohistochemistry analysis of Human prostate tissue labeling Rac1 with ab155938 at 1/50. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rac1 antibody (ab155938)Immunohistochemistry analysis of Human tonsil tissue labeling Rac1 with ab155938 at 1/50.