Anti-RASGRP3 (phospho S172) antibody [EPNCIR149] - BSA and Azide free (ab249371)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPNCIR149] to RASGRP3 (phospho S172) - BSA and Azide free
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-RASGRP3 (phospho S172) antibody [EPNCIR149] - BSA and Azide free
See all RASGRP3 primary antibodies -
Description
Rabbit monoclonal [EPNCIR149] to RASGRP3 (phospho S172) - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human -
Immunogen
Synthetic peptide within Human RASGRP3. The exact sequence is proprietary.
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General notes
ab249371 is the carrier-free version of ab166887 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab249371 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This antibody was developed as part of a collaboration between Epitomics, the National Cancer Institute's Center for Cancer Research and the lab of Peter Blumberg. Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPNCIR149 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-RASGRP3 (phospho S172) antibody [EPNCIR149] (ab166887) at 1/1000 dilution (purified)
Lane 1 : LnCap wild type cell lysate transfected with RASGRP3 + Go6983
Lane 2 : LnCap wild type cell lysate, untransfected
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 78 kDa
Observed band size: 78 kDaThis data was developed using ab166887, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-RASGRP3 (phospho S172) antibody [EPNCIR149] (ab166887) at 1/1000 dilution
Lane 1 : LNCap wild type cell lysate untransfected
Lane 2 : LNCap wild type cell lysate transfected with RASGRP3
Lane 3 : LNCap wild type cell lysate transfected with RASGRP3 + PMA
Lane 4 : LNCap wild type cell lysate transfected with RASGRP3 + Go6983
Lane 5 : LNCap wild type cell lysate transfected with RASGRP3 + Go6983 + PMA
Lane 6 : LNCap wild type cell lysate transfected with s172A mutant RASGRP3
Lane 7 : LNCap wild type cell lysate transfected with s172A mutant RASGRP3 + PMA
Lane 8 : LNCap wild type cell lysate transfected with s172A mutant RASGRP3 + Go6983
Lane 9 : LNCap wild type cell lysate transfected with s172A mutant RASGRP3 + Go6983 + PMA
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 78 kDaThis data was developed using ab166887, the same antibody clone in a different buffer formulation.
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