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Signal Transduction Signaling Pathway G Protein Signaling Small G Proteins Ras Family

Anti-RASGRP3 (phospho S172) antibody [EPNCIR149] - BSA and Azide free (ab249371)

Anti-RASGRP3 (phospho S172) antibody [EPNCIR149] - BSA and Azide free (ab249371)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPNCIR149] to RASGRP3 (phospho S172) - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Human

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Overview

  • Product name

    Anti-RASGRP3 (phospho S172) antibody [EPNCIR149] - BSA and Azide free
    See all RASGRP3 primary antibodies
  • Description

    Rabbit monoclonal [EPNCIR149] to RASGRP3 (phospho S172) - BSA and Azide free
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human RASGRP3. The exact sequence is proprietary.

  • General notes

    ab249371 is the carrier-free version of ab166887 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab249371 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This antibody was developed as part of a collaboration between Epitomics, the National Cancer Institute's Center for Cancer Research and the lab of Peter Blumberg. Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPNCIR149
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • Small G Proteins
    • Ras Family
    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • GPCR

Images

  • Western blot - Anti-RASGRP3 (phospho S172) antibody [EPNCIR149] - BSA and Azide free (ab249371)
    Western blot - Anti-RASGRP3 (phospho S172) antibody [EPNCIR149] - BSA and Azide free (ab249371)
    All lanes : Anti-RASGRP3 (phospho S172) antibody [EPNCIR149] (ab166887) at 1/1000 dilution (purified)

    Lane 1 : LnCap wild type cell lysate transfected with RASGRP3 + Go6983
    Lane 2 : LnCap wild type cell lysate, untransfected

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 78 kDa
    Observed band size: 78 kDa



    This data was developed using ab166887, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-RASGRP3 (phospho S172) antibody [EPNCIR149] - BSA and Azide free (ab249371)
    Western blot - Anti-RASGRP3 (phospho S172) antibody [EPNCIR149] - BSA and Azide free (ab249371)
    All lanes : Anti-RASGRP3 (phospho S172) antibody [EPNCIR149] (ab166887) at 1/1000 dilution

    Lane 1 : LNCap wild type cell lysate untransfected
    Lane 2 : LNCap wild type cell lysate transfected with RASGRP3
    Lane 3 : LNCap wild type cell lysate transfected with RASGRP3 + PMA
    Lane 4 : LNCap wild type cell lysate transfected with RASGRP3 + Go6983
    Lane 5 : LNCap wild type cell lysate transfected with RASGRP3 + Go6983 + PMA
    Lane 6 : LNCap wild type cell lysate transfected with s172A mutant RASGRP3
    Lane 7 : LNCap wild type cell lysate transfected with s172A mutant RASGRP3 + PMA
    Lane 8 : LNCap wild type cell lysate transfected with s172A mutant RASGRP3 + Go6983
    Lane 9 : LNCap wild type cell lysate transfected with s172A mutant RASGRP3 + Go6983 + PMA

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 78 kDa



    This data was developed using ab166887, the same antibody clone in a different buffer formulation.

     

  • Anti-RASGRP3 (phospho S172) antibody [EPNCIR149] - BSA and Azide free (ab249371)
    Anti-RASGRP3 (phospho S172) antibody [EPNCIR149] - BSA and Azide free (ab249371)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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