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Anti-Rab5 antibody [3A4] - Early Endosome Marker (ab66746)

Key features and details

  • Mouse monoclonal [3A4] to Rab5 - Early Endosome Marker
  • Suitable for: WB, ICC/IF
  • Knockout validated
  • Reacts with: Mouse, Human
  • Isotype: IgG2b

Overview

  • Product name

    Anti-Rab5 antibody [3A4] - Early Endosome Marker
    See all Rab5 primary antibodies

  • Description

    Mouse monoclonal [3A4] to Rab5 - Early Endosome Marker

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment corresponding to amino acids 1-215 of Human Rab5 purified from E. coli.

  • Positive control

    • WB: Extracts of mouse and human brain. ICC/IF: U87MG cells.

  • General notes

    This product was changed from ascites to tissue culture supernatant on 24/01/2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Properties

Images

  • Western blot - Anti-Rab5 antibody [3A4] - Drosophila Early Endosome Marker (ab66746)
    Western blot - Anti-Rab5 antibody [3A4] - Drosophila Early Endosome Marker (ab66746)

    Lane 1: Wild type HAP1 whole cell lysate (20 µg)
    Lane 2: Rab5 knockout HAP1 whole cell lysate (20 µg)
    Lane 3: Hu brain whole cell lysate (20 µg)
    Lane 4: Ms brain whole cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab66746 observed at 25 kDa. Red - loading control, ab181602, observed at 37 kDa.

    This image was produced using the ascites version of this antibody. 

  • Immunocytochemistry/ Immunofluorescence - Anti-Rab5 antibody [3A4] - Drosophila Early Endosome Marker (ab66746)
    Immunocytochemistry/ Immunofluorescence - Anti-Rab5 antibody [3A4] - Drosophila Early Endosome Marker (ab66746)
    Immunocytochemistry/ Immunofluorescence analysis of Rab5a in U87MG cells. The cell was stained with ab66746 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
  • Western blot - Anti-Rab5 antibody [3A4] - Drosophila Early Endosome Marker (ab66746)
    Western blot - Anti-Rab5 antibody [3A4] - Drosophila Early Endosome Marker (ab66746)
    Anti-Rab5 antibody [3A4] - Early Endosome Marker (ab66746) at 1/500 dilution + mouse brain at 45 µg

    Secondary
    goat anti-mouse secondary antibody conjugated to HRP

    Predicted band size: 24 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Rab5 antibody [3A4] - Drosophila Early Endosome Marker (ab66746)
    Immunocytochemistry/ Immunofluorescence - Anti-Rab5 antibody [3A4] - Drosophila Early Endosome Marker (ab66746)

    ab66746 staining Rab5 in the U87MG human cell line at a dilution of 1/100. The cells were fixed before staining with a mixture of acetone and methanol, 1:1. DAPI was used fhor nuclear staining.

    This image was produced using the ascites version of this antibody. 

  • Western blot - Anti-Rab5 antibody [3A4] - Drosophila Early Endosome Marker (ab66746)
    Western blot - Anti-Rab5 antibody [3A4] - Drosophila Early Endosome Marker (ab66746)
    Anti-Rab5 antibody [3A4] - Early Endosome Marker (ab66746) at 1/500 dilution + extracts of mouse brain at 45 µg

    Secondary
    HRP-conjugated goat anti-mouse

    Developed using the ECL technique.

    Predicted band size: 24 kDa
    Observed band size: 24 kDa



    This image was produced using the ascites version of this antibody. 

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