Anti-Rab5 antibody - C-terminal (ab228770)
Key features and details
- Rabbit polyclonal to Rab5 - C-terminal
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Rab5 antibody - C-terminal
See all Rab5 primary antibodies -
Description
Rabbit polyclonal to Rab5 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Dog, Rhesus monkey
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Immunogen
Synthetic peptide within Human Rab5 (C terminal). The exact sequence is proprietary. (Carrier-protein conjugated).
Database link: P20339 -
Positive control
- WB: A431 and Raji whole cell lysate; Mouse brain lysate. IP: HeLa whole cell lysate. IHC-P: U-87 MG xenograft.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Rab5 antibody - C-terminal (ab228770)All lanes : Anti-Rab5 antibody - C-terminal (ab228770) at 1/1000 dilution
Lane 1 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 2 : Raji (human Burkitt's lymphoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 24 kDa12% SDS-PAGE gel.
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Immunoprecipitation - Anti-Rab5 antibody - C-terminal (ab228770)Rab5 was immunoprecipitated from 1 mg of HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab228770. Western blot was performed from the immunoprecipitate using ab228770 at 1/1000 dilution. Anti-rabbit IgG was used as secondary antibody.
Lane 1: HeLa whole cell lysate 30 µg (Input).
Lane 2: Control IP with 2 μg of preimmune rabbit IgG in HeLa whole cell lysate.
Lane 3: ab228770 IP in HeLa whole cell lysate.
15% SDS-PAGE gel.
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Western blot - Anti-Rab5 antibody - C-terminal (ab228770)Anti-Rab5 antibody - C-terminal (ab228770) at 1/1000 dilution + Mouse brain lysate at 50 µg
Secondary
HRP-conjugated anti-rabbit IgG
Predicted band size: 24 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab5 antibody - C-terminal (ab228770)Paraffin-embedded U-87 MG (human glioblastoma-astrocytoma epithelial cell line) xenograft stained for Rab5 using ab228770 at 1/500 dilution in immunohistochemical analysis.
