Anti-Rab5 antibody - Drosophila Early Endosome Marker (ab31261)
Key features and details
- Rabbit polyclonal to Rab5 - Drosophila Early Endosome Marker
- Suitable for: WB
- Reacts with: Drosophila melanogaster
- Isotype: IgG
Overview
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Product name
Anti-Rab5 antibody - Drosophila Early Endosome Marker
See all Rab5 primary antibodies -
Description
Rabbit polyclonal to Rab5 - Drosophila Early Endosome Marker -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Drosophila melanogaster -
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 200 to the C-terminus of Fruit fly (Drosophila melanogaster) Rab5.
Read Abcam's proprietary immunogen policy (Peptide available as ab32489.)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab31261 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa). Target
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Function
Required for the fusion of plasma membranes and early endosomes. -
Sequence similarities
Belongs to the small GTPase superfamily. Rab family. -
Cellular localization
Cell membrane. Early endosome membrane. Melanosome. Enriched in stage I melanosomes. - Information by UniProt
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Alternative names
- RAB 5 antibody
- RAB 5A antibody
- RAB5A antibody
see all
Images
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Western blot - Anti-Rab5 antibody - Drosophila Early Endosome Marker (ab31261)Anti-Rab5 antibody - Drosophila Early Endosome Marker (ab31261) at 1 µg/ml +
Schneider L2 whole cell lysate (ab14893) at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 26 kDa
Observed band size: 26 kDa
Additional bands at: 135 kDa, 145 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutes
ab31261 detects a strong 26 kDa band in Drosophila lysate consistent with expected molecular weight of Rab5. In some batches of this antibody, we have observed additional bands at ~135, 145 and 70 kDa, the identity of which are unknown. -
Immunocytochemistry/ Immunofluorescence - Anti-Rab5 antibody - Drosophila Early Endosome Marker (ab31261)Image from Huang HR et al, Proc Natl Acad Sci U S A. 2010 May 4;107(18):8322-7. Epub 2010 Apr 19, Fig S2. DOI 10.1073/pnas.1004031107ab31261 staining Rab5 in Drosophila S2 cells by Immunocytochemistry/ Immunofluorescence.
S2 cells were fixed in 4% paraformaldehyde for 10 minutes and then permeablized by incubating with 0.2% Triton X-100 in PBS for 5 minutes at room temperature. After blocking, cells were incubated overnight with ab31261 diluted in PBS containing 10% horse serum and 0.2% Triton X-100. A Fluorescein-conjugated anti-rabbit IgG was used as the secondary.
Protocols
Datasheets and documents
References (40)
ab31261 has been referenced in 40 publications.
- Bergkvist L et al. Mapping pathogenic processes contributing to neurodegeneration in Drosophila models of Alzheimer's disease. FEBS Open Bio 10:338-350 (2020). PubMed: 31823504
- Lin B et al. Collectively stabilizing and orienting posterior migratory forces disperses cell clusters in vivo. Nat Commun 11:4477 (2020). PubMed: 32901019
- Gualtieri M & Vaccari T Genetic and Cell Biology Methods to Study ESCRTs in Drosophila melanogaster. Methods Mol Biol 1998:13-29 (2019). PubMed: 31250291
- Lorincz P et al. Vps8 overexpression inhibits HOPS-dependent trafficking routes by outcompeting Vps41/Lt. Elife 8:N/A (2019). PubMed: 31194677
- Negreiros E et al. N-linked glycosylation restricts the function of Short gastrulation to bind and shuttle BMPs. Development 145:N/A (2018). PubMed: 30355725
Images
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Western blot - Anti-Rab5 antibody - Drosophila Early Endosome Marker (ab31261)Anti-Rab5 antibody - Drosophila Early Endosome Marker (ab31261) at 1 µg/ml +
Schneider L2 whole cell lysate (ab14893) at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 26 kDa
Observed band size: 26 kDa
Additional bands at: 135 kDa, 145 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutes
ab31261 detects a strong 26 kDa band in Drosophila lysate consistent with expected molecular weight of Rab5. In some batches of this antibody, we have observed additional bands at ~135, 145 and 70 kDa, the identity of which are unknown. -
Immunocytochemistry/ Immunofluorescence - Anti-Rab5 antibody - Drosophila Early Endosome Marker (ab31261) Image from Huang HR et al, Proc Natl Acad Sci U S A. 2010 May 4;107(18):8322-7. Epub 2010 Apr 19, Fig S2. DOI 10.1073/pnas.1004031107ab31261 staining Rab5 in Drosophila S2 cells by Immunocytochemistry/ Immunofluorescence.
S2 cells were fixed in 4% paraformaldehyde for 10 minutes and then permeablized by incubating with 0.2% Triton X-100 in PBS for 5 minutes at room temperature. After blocking, cells were incubated overnight with ab31261 diluted in PBS containing 10% horse serum and 0.2% Triton X-100. A Fluorescein-conjugated anti-rabbit IgG was used as the secondary.
