Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-QKI antibody [EPR7306] (ab126742)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7306] to QKI
  • Suitable for: WB, IP, ICC/IF
  • Knockout validated
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-QKI antibody [EPR7306]
    See all QKI primary antibodies

  • Description

    Rabbit monoclonal [EPR7306] to QKI

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Mouse
    IP
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human QKI aa 250 to the C-terminus. The exact sequence is proprietary.
    Database link: Q96PU8

  • Positive control

    • WB: HAP1, Neuro 2a, HeLa and K562 whole cell lysates. IP: HeLa whole cell lysate. ICC/IF: Neuro-2a cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-QKI antibody [EPR7306] (ab126742)
    Western blot - Anti-QKI antibody [EPR7306] (ab126742)
    All lanes : Anti-QKI antibody [EPR7306] (ab126742) at 1/1000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : QKI knockout HAP1 whole cell lysate
    Lane 3 : HeLa whole cell lysate
    Lane 4 : THP-1 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 37 kDa



    Lanes 1 - 4: Merged signal (red and green). Green - ab126742 observed at 37 kDa. Red - loading control, ab7291, observed at 50 kDa.

    ab126742 was shown to specifically react with in wild-type HAP1 cells as signal was lost in QKI knockout cells. Wild-type and QKI knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% NF Milk. ab126742 and ab7291 (Mouse anti Tubulin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-QKI antibody [EPR7306] (ab126742)
    Immunocytochemistry/ Immunofluorescence - Anti-QKI antibody [EPR7306] (ab126742)

    Immunocytochemistry/ Immunofluorescence analysis of Neuro-2a (Mouse neuroblastoma neuroblast) cells labeling QKI with Purified ab126742 at 1:100 dilution (5.6μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Immunoprecipitation - Anti-QKI antibody [EPR7306] (ab126742)
    Immunoprecipitation - Anti-QKI antibody [EPR7306] (ab126742)

    ab126742 (purified) at 1:30 dilution (2µg) immunoprecipitating QKI in HeLa whole cell lysate.
    Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10µg
    Lane 2 (+): ab126742 & HeLa whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab126742 in HeLa whole cell lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

  • Western blot - Anti-QKI antibody [EPR7306] (ab126742)
    Western blot - Anti-QKI antibody [EPR7306] (ab126742)
    All lanes : Anti-QKI antibody [EPR7306] (ab126742) at 1/2000 dilution (purified)

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
    Lane 2 : Neuro-2a (Mouse neuroblastoma neuroblast) whole cell lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 37 kDa



    Blocking and diluting buffer : 5% NFDM/TBST
  • Western blot - Anti-QKI antibody [EPR7306] (ab126742)
    Western blot - Anti-QKI antibody [EPR7306] (ab126742)
    All lanes : Anti-QKI antibody [EPR7306] (ab126742) at 1/1000 dilution (unpurified)

    Lane 1 : Neuro 2a cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : K562 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat-anti-rabbit HRP at 1/2000 dilution

    Developed using the ECL technique.

    Predicted band size: 37 kDa
    Observed band size: 38 kDa
    why is the actual band size different from the predicted?

  • OI-RD Scanning - Anti-QKI antibody [EPR7306] (ab126742)
    OI-RD Scanning - Anti-QKI antibody [EPR7306] (ab126742)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-QKI antibody [EPR7306] (ab126742)
    Anti-QKI antibody [EPR7306] (ab126742)

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