Anti-QKI antibody [N147/6] (ab186245)
![Anti-QKI antibody [N147/6] (ab186245)](https://www.abcam.com/ps/products/186/ab186245/Images/ab186245-368835-anti-qki-antibody-n1476-western-blot-knockout-hap1-empty-qki-ko-hela-thp1-human.jpg "Anti-QKI antibody [N147/6] (ab186245)")
Key features and details
- Mouse monoclonal [N147/6] to QKI
- Suitable for: WB, ICC/IF
- Knockout validated
- Reacts with: Mouse, Rat, Human
- Isotype: IgG2b
Overview
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Product name
Anti-QKI antibody [N147/6]
See all QKI primary antibodies -
Description
Mouse monoclonal [N147/6] to QKI -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB RatHuman
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Immunogen
Fusion protein corresponding to Human QKI aa 1 to the C-terminus.
Database link: Q96PU8 -
Positive control
- WB: Rat brain membrane lysate; HAP1 whole cell lysate; HeLa whole cell lysate; THP-1 whole cell lysate. ICC/IF: SK-N-BE cells.
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General notes
The clone number has been updated from S147-6 to N147/6, both clone numbers name the same antibody clone.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
N147/6 -
Isotype
IgG2b -
Research areas
Images
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Western blot - Anti-QKI antibody [N147/6] (ab186245)All lanes : Anti-QKI antibody [N147/6] (ab186245) at 1/1000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : QKI knockout HAP1 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : THP-1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 37 kDa
Observed band size: 39 kDa why is the actual band size different from the predicted?Lanes 1 - 4: Merged signal (red and green). Green - ab186245 observed at 39 kDa. Red - loading control, ab52866, observed at 50 kDa.
ab186245 was shown to specifically react with in wild-type HAP1 cells as signal was lost in QKI knockout cells. Wild-type and QKI knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% NF Milk. Ab186245 and ab52866 (Rabbit anti-tubulin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Immunocytochemistry/ Immunofluorescence - Anti-QKI antibody [N147/6] (ab186245)](https://www.abcam.com/ps/products/186/ab186245/Images/ab186245-312169-anti-qki-antibody-n147-6-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-QKI antibody [N147/6] (ab186245)SK-N-BE (Human neuroblastoma cell line) cells stained for QKI using ab186245 at a dilution of 1/100 in ICC/IF.
Cells were fixed in 4% formaldehyde for 15 minutes at RT.
Secondary used is a Goat Anti-Mouse ATTO 488 at 1:100 dilution for 60 minutes at RT.
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![Western blot - Anti-QKI antibody [N147/6] (ab186245)](https://www.abcam.com/ps/products/186/ab186245/Images/ab186245-312170-anti-qki-antibody-n147-6-western-blot.jpg)
Western blot - Anti-QKI antibody [N147/6] (ab186245)Anti-QKI antibody [N147/6] (ab186245) at 1/200 dilution + Rat brain membrane lysate at 15 µg
Secondary
Goat Anti-Mouse IgG: HRP at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 37 kDa
Exposure time: 6 minutesBlock: 2% BSA and 2% Skimmed milk in 1X TBST.
Primary antibody incubated for 16 hours at +4°C.
Secondary antibody incubated for 1 hour at RT.
