Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-QKI antibody [EPR7306] - BSA and Azide free (ab232502)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7306] to QKI - BSA and Azide free
  • Suitable for: WB, IP, ICC/IF
  • Knockout validated
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-QKI antibody [EPR7306] - BSA and Azide free
    See all QKI primary antibodies

  • Description

    Rabbit monoclonal [EPR7306] to QKI - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IP, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HAP1, HeLa, and THP-1 whole cell lysates. ICC/IF: Neuro-2a cells.

  • General notes

    Ab232502 is the carrier-free version of ab126742. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab232502 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Western blot - Anti-QKI antibody [EPR7306] - BSA and Azide free (ab232502)
    Western blot - Anti-QKI antibody [EPR7306] - BSA and Azide free (ab232502)
    All lanes : Anti-QKI antibody [EPR7306] (ab126742) at 1/1000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : QKI knockout HAP1 whole cell lysate
    Lane 3 : HeLa whole cell lysate
    Lane 4 : THP-1 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 37 kDa



    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab126742).

    Lanes 1 - 4: Merged signal (red and green). Green - ab126742 observed at 37 kDa. Red - loading control, ab7291, observed at 50 kDa.

    ab126742 was shown to specifically react with in wild-type HAP1 cells as signal was lost in QKI knockout cells. Wild-type and QKI knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% NF Milk. ab126742 and ab7291 (Mouse anti Tubulin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Immunoprecipitation - Anti-QKI antibody [EPR7306] - BSA and Azide free (ab232502)
    Immunoprecipitation - Anti-QKI antibody [EPR7306] - BSA and Azide free (ab232502)

    ab126742 (purified) at 1:30 dilution (2µg) immunoprecipitating QKI in HeLa whole cell lysate.
    Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10µg
    Lane 2 (+): ab126742 & HeLa whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab126742 in HeLa whole cell lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab126742).

  • OI-RD Scanning - Anti-QKI antibody [EPR7306] - BSA and Azide free (ab232502)
    OI-RD Scanning - Anti-QKI antibody [EPR7306] - BSA and Azide free (ab232502)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab126742).

  • Immunocytochemistry/ Immunofluorescence - Anti-QKI antibody [EPR7306] - BSA and Azide free (ab232502)
    Immunocytochemistry/ Immunofluorescence - Anti-QKI antibody [EPR7306] - BSA and Azide free (ab232502)

    Immunofluorescent analysis of Neuro-2a (Mouse neuroblastoma neuroblast) cells labeling QKI with Purified ab126742 at 1:100 dilution (5.6μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab126742).

  • Anti-QKI antibody [EPR7306] - BSA and Azide free (ab232502)
    Anti-QKI antibody [EPR7306] - BSA and Azide free (ab232502)

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