Anti-Prostaglandin D2 Receptor antibody (ab99446)
Key features and details
- Rabbit polyclonal to Prostaglandin D2 Receptor
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Prostaglandin D2 Receptor antibody
See all Prostaglandin D2 Receptor primary antibodies -
Description
Rabbit polyclonal to Prostaglandin D2 Receptor -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseHuman
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- This antibody gave a positive signal in the following whole cell lysates: NIH3T3; EL4; Raw 264.7; A549; HepG2 and in the following tissue lysates: Mouse lung; Mouse brain; Mouse kidney; Rat small intestine; Rat kidney.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab99446 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB MouseHumanApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).Target
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Function
Receptor for prostaglandin D2 (PGD2). The activity of this receptor is mainly mediated by G(s) proteins that stimulate adenylate cyclase, resulting in an elevation of intracellular cAMP. A mobilization of calcium is also observed, but without formation of inositol 1,4,5-trisphosphate. -
Tissue specificity
Expressed in retinal choroid, ciliary epithelium, longitudinal and circular ciliary muscles, iris, small intestine and platelet membranes. -
Involvement in disease
Genetic variations in PTGDR are associated with susceptibility to asthma-related traits type 1 (ASRT1) [MIM:607277]. Asthma-related traits include clinical symptoms of asthma, such as coughing, wheezing and dyspnea. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 5729 Human
- Entrez Gene: 19214 Mouse
- Entrez Gene: 498475 Rat
- Omim: 604687 Human
- SwissProt: Q13258 Human
- SwissProt: P70263 Mouse
- SwissProt: Q9R261 Rat
- Unigene: 306831 Human
see all -
Alternative names
- AS 1 antibody
- AS1 antibody
- ASRT 1 antibody
see all
Images
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Western blot - Anti-Prostaglandin D2 Receptor antibody (ab99446)All lanes : Anti-Prostaglandin D2 Receptor antibody (ab99446) at 1 µg/ml
Lane 1 :Mouse lung normal tissue lysate - total protein (ab29297)
Lane 2 : Brain (Mouse) Tissue Lysate
Lane 3 : Kidney (Mouse) Tissue Lysate
Lane 4 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 5 :EL4 whole cell lysate (ab7183)
Lane 6 : RAW 264.7 (Mouse leukaemic monocyte macrophage cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 40 kDa
Observed band size: 40 kDa
Additional bands at: 87 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutes -
Western blot - Anti-Prostaglandin D2 Receptor antibody (ab99446)All lanes : Anti-Prostaglandin D2 Receptor antibody (ab99446) at 1 µg/ml
Lane 1 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 3 : Small Intestine (Rat) Tissue Lysate
Lane 4 : Kidney (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 40 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
Additional bands at: 95 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minute
Datasheets and documents
References (3)
ab99446 has been referenced in 3 publications.
- Sun LQ et al. Effects of MicroRNA-592-5p on Hippocampal Neuron Injury Following Hypoxic-Ischemic Brain Damage in Neonatal Mice - Involvement of PGD2/DP and PTGDR. Cell Physiol Biochem 45:458-473 (2018). PubMed: 29402808
- Guan NN et al. Prostaglandin D2 effects and DP1 /DP2 receptor distribution in guinea pig urinary bladder out-flow region. J Cell Mol Med 21:234-243 (2017). IHC . PubMed: 27664012
- Guan NN et al. Receptors involved in the modulation of guinea pig urinary bladder motility by prostaglandin D2. Br J Pharmacol 172:4024-37 (2015). PubMed: 25917171
Images
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Western blot - Anti-Prostaglandin D2 Receptor antibody (ab99446)All lanes : Anti-Prostaglandin D2 Receptor antibody (ab99446) at 1 µg/ml
Lane 1 :Mouse lung normal tissue lysate - total protein (ab29297)
Lane 2 : Brain (Mouse) Tissue Lysate
Lane 3 : Kidney (Mouse) Tissue Lysate
Lane 4 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 5 :EL4 whole cell lysate (ab7183)
Lane 6 : RAW 264.7 (Mouse leukaemic monocyte macrophage cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 40 kDa
Observed band size: 40 kDa
Additional bands at: 87 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutes -
Western blot - Anti-Prostaglandin D2 Receptor antibody (ab99446)All lanes : Anti-Prostaglandin D2 Receptor antibody (ab99446) at 1 µg/ml
Lane 1 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 3 : Small Intestine (Rat) Tissue Lysate
Lane 4 : Kidney (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 40 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
Additional bands at: 95 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minute
