Anti-PRA1 antibody [EPR1747Y] (ab76413)
![Anti-PRA1 antibody [EPR1747Y] (ab76413)](https://www.abcam.com/ps/products/76/ab76413/Images/ab76413-389442-anti-pra1-antibody-epr1747y-western-blot-human-knockout-lysate.jpg "Anti-PRA1 antibody [EPR1747Y] (ab76413)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1747Y] to PRA1
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-PRA1 antibody [EPR1747Y]
See all PRA1 primary antibodies -
Description
Rabbit monoclonal [EPR1747Y] to PRA1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide within Human PRA1 (C terminal). The exact sequence is proprietary.
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Positive control
- WB: HeLa and HEK-293T cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR1747Y -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PRA1 antibody [EPR1747Y] (ab76413)All lanes : Anti-PRA1 antibody [EPR1747Y] (ab76413) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : RABAC1 knockout HeLa cell lysate
Lane 3 : HEK293 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/20000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDaLanes 1-3: Merged signal (red and green). Green - ab76413 observed at 21 kDa. Red - loading control ab7291 observed at 50 kDa.
ab76413 Anti-PRA1 antibody [EPR1747Y] was shown to specifically react with PRA1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265702 (knockout cell lysate ab258162) was used. Wild-type and PRA1 knockout samples were subjected to SDS-PAGE. ab76413 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-PRA1 antibody [EPR1747Y] (ab76413)](https://www.abcam.com/ps/products/76/ab76413/Images/ab76413-91814-anti-pra1-antibody-epr1747y-western-blot.jpg)
Western blot - Anti-PRA1 antibody [EPR1747Y] (ab76413)Anti-PRA1 antibody [EPR1747Y] (ab76413) at 1/2000 dilution + 293 cell lysate at 10 µg
Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDa
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![Anti-PRA1 antibody [EPR1747Y] (ab76413)](https://www.abcam.com/ps/products/76/ab76413/Images/ab76413-3-benefits-of-recombinant-antibodies.png)
Anti-PRA1 antibody [EPR1747Y] (ab76413)
