Antibodies, Proteins, Kits and Reagents for Life Science

328 339 ₸ Product size

100 µl 328 339 ₸ 10 µl 110 563 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Poliovirus Receptor/PVR antibody [EPR22672-151] (ab267788)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR22672-151] to Poliovirus Receptor/PVR
Suitable for: WB, IHC-P, Flow Cyt
Knockout validated
Reacts with: Human

Product name

Anti-Poliovirus Receptor/PVR antibody [EPR22672-151]
See all Poliovirus Receptor/PVR primary antibodies
Description

Rabbit monoclonal [EPR22672-151] to Poliovirus Receptor/PVR
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- IHC-P: Human breast cancer, lung cancer and colon cancer tissue. Flow Cyt: Raji cells. WB: HEK293T, A549, WI-38, HT-1080, U-87 MG, HUVEC and MCF7 whole cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR22672-151
Isotype

IgG
Research areas

Flow Cytometry - Anti-Poliovirus Receptor/PVR antibody [EPR22672-151] (ab267788)

Flow cytometric analysis of Raji (Human Burkitt's lymphoma B lymphocyte) (Left panel) and A549 (Human lung carcinoma epithelial cell) (Right panel) labeling Poliovirus Receptor/PVR with ab267788 at 1/50 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

Negative control: Raji (PMID:31500665).

Gated on viable cells.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Poliovirus Receptor/PVR antibody [EPR22672-151] (ab267788)

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue labeling Poliovirus Receptor/PVR with ab267788 at 1/500 dilution (1.01µg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in human lung cancer (PMID:20331633). Counterstained with hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Western blot - Anti-Poliovirus Receptor/PVR antibody [EPR22672-151] (ab267788)

All lanes : Anti-Poliovirus Receptor/PVR antibody [EPR22672-151] (ab267788) at 1/1000 dilution

Lane 1 : Wild-type HEK-293T cell lysate
Lane 2 : PVR knockout HEK-293T cell lysate
Lane 3 : Wild-type A549 cell lysate
Lane 4 : PVR knockout A549 cell lysate

Lysates/proteins at 20 µg per lane.

Performed under reducing conditions.

Predicted band size: 45 kDa
Observed band size: 70 kDa
why is the actual band size different from the predicted?

Lanes 1- 4: Merged signal (red and green). Green - ab267788 observed at 70 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

ab267788 was shown to react with Poliovirus Receptor/PVR in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab266102 (knockout cell lysate ab257622) was used. Wild-type HEK-293T and PVR knockout HEK-293T cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab267788 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Poliovirus Receptor/PVR antibody [EPR22672-151] (ab267788)

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue labeling Poliovirus Receptor/PVR with ab267788 at 1/500 dilution (1.01µg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in human colon cancer (PMID: 28816021,11454801). Counterstained with hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Poliovirus Receptor/PVR antibody [EPR22672-151] (ab267788)

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue labeling Poliovirus Receptor/PVR with ab267788 at 1/500 dilution (1.01µg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in human breast cancer (PMID:31035013). Counterstained with hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Western blot - Anti-Poliovirus Receptor/PVR antibody [EPR22672-151] (ab267788)

All lanes : Anti-Poliovirus Receptor/PVR antibody [EPR22672-151] (ab267788) at 1/1000 dilution

Lane 1 : A549 (human lung carcinoma epithelial cell), whole cell lysate
Lane 2 : WI-38 (human fetal lung fibroblast), whole cell lysate
Lane 3 : HT-1080 (human fibrosarcoma epithelial cell), whole cell lysate
Lane 4 : U-87 MG (human glioblastoma-astrocytoma epithelial cell), whole cell lysate
Lane 5 : HUVEC (human umbilical vein endothelial cell), whole cell lysate
Lane 6 : Raji (human Burkitt's lymphoma B lymphocyte), whole cell lysate
Lane 7 : MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 45 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure time: 10 seconds.

Negative control: Raji (PMID: 28829151, 31500665).

PVR/CD155 is a glycoprotein. The molecular weight observed is consistent with what has been described in the literature (PMID:17893876,11454801).
Western blot - Anti-Poliovirus Receptor/PVR antibody [EPR22672-151] (ab267788)

All lanes : Anti-Poliovirus Receptor/PVR antibody [EPR22672-151] (ab267788) at 1/1000 dilution

Lane 1 : Human placenta lysate
Lane 2 : Human lung cancer lysate
Lane 3 : Human colon cancer lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/2000 dilution

Predicted band size: 45 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure time: 3 minutes.

PVR/CD155 is a glycoprotein. The molecular weight observed is consistent with what has been described in the literature (PMID:17893876,11454801).

This blot was developed using a higher sensitivity ECL substrate.
Anti-Poliovirus Receptor/PVR antibody [EPR22672-151] (ab267788)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com