Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Poliovirus Receptor/PVR antibody [EPR22672-151] - BSA and Azide free (ab267794)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22672-151] to Poliovirus Receptor/PVR - BSA and Azide free
  • Suitable for: WB, IHC-P, Flow Cyt
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-Poliovirus Receptor/PVR antibody [EPR22672-151] - BSA and Azide free
    See all Poliovirus Receptor/PVR primary antibodies

  • Description

    Rabbit monoclonal [EPR22672-151] to Poliovirus Receptor/PVR - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details
    Unsuitable for: ICC/IF or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human breast cancer, lung cancer and colon cancer tissue. Flow Cyt: Raji cells. WB: HEK293T, A549, WI-38, HT-1080, U-87 MG, HUVEC and MCF7 whole cell lysate.

  • General notes

    ab267794 is the carrier-free version of ab267788. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab267794 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR22672-151

  • Isotype

    IgG

  • Research areas

Images

  • Flow Cytometry - Anti-Poliovirus Receptor/PVR antibody [EPR22672-151] - BSA and Azide free (ab267794)
    Flow Cytometry - Anti-Poliovirus Receptor/PVR antibody [EPR22672-151] - BSA and Azide free (ab267794)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab267788).

    Flow cytometric analysis of Raji (Human Burkitt's lymphoma B lymphocyte) (Left panel) and A549 (Human lung carcinoma epithelial cell) (Right panel) labeling Poliovirus Receptor/PVR with ab267788 at 1/50 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    Negative control: Raji (PMID:31500665).

    Gated on viable cells.

     

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab267788).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Poliovirus Receptor/PVR antibody [EPR22672-151] - BSA and Azide free (ab267794)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Poliovirus Receptor/PVR antibody [EPR22672-151] - BSA and Azide free (ab267794)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue labeling Poliovirus Receptor/PVR with ab267788 at 1/500 dilution (1.01µg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in human lung cancer (PMID:20331633). Counterstained with hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab267788).

  • Western blot - Anti-Poliovirus Receptor/PVR antibody [EPR22672-151] - BSA and Azide free (ab267794)
    Western blot - Anti-Poliovirus Receptor/PVR antibody [EPR22672-151] - BSA and Azide free (ab267794)
    All lanes : Anti-Poliovirus Receptor/PVR antibody [EPR22672-151] (ab267788) at 1/1000 dilution

    Lane 1 : Wild-type HEK-293T cell lysate
    Lane 2 : PVR knockout HEK-293T cell lysate
    Lane 3 : Wild-type A549 cell lysate
    Lane 4 : PVR knockout A549 cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 45 kDa
    Observed band size: 70 kDa
    why is the actual band size different from the predicted?



    This data was developed using the same antibody clone in a different buffer formulation (ab267788).

      Lanes 1- 4: Merged signal (red and green). Green - ab267788 observed at 70 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

     ab267788 was shown to react with Poliovirus Receptor/PVR in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab266102 (knockout cell lysate ab257622) was used. Wild-type HEK-293T and PVR knockout HEK-293T cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab267788 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Poliovirus Receptor/PVR antibody [EPR22672-151] - BSA and Azide free (ab267794)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Poliovirus Receptor/PVR antibody [EPR22672-151] - BSA and Azide free (ab267794)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue labeling Poliovirus Receptor/PVR with ab267788 at 1/500 dilution (1.01µg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in human colon cancer (PMID: 28816021,11454801). Counterstained with hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab267788).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Poliovirus Receptor/PVR antibody [EPR22672-151] - BSA and Azide free (ab267794)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Poliovirus Receptor/PVR antibody [EPR22672-151] - BSA and Azide free (ab267794)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue labeling Poliovirus Receptor/PVR with ab267788 at 1/500 dilution (1.01µg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in human breast cancer (PMID:31035013). Counterstained with hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab267788).

  • Anti-Poliovirus Receptor/PVR antibody [EPR22672-151] - BSA and Azide free (ab267794)
    Anti-Poliovirus Receptor/PVR antibody [EPR22672-151] - BSA and Azide free (ab267794)

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