Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CREB3 antibody [EPR14215(B)] (ab180119)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14215(B)] to CREB3
  • Suitable for: ICC/IF, Flow Cyt, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-CREB3 antibody [EPR14215(B)]
    See all CREB3 primary antibodies

  • Description

    Rabbit monoclonal [EPR14215(B)] to CREB3

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human CREB3 aa 50-150. The exact sequence is proprietary.
    Database link: O43889

  • Positive control

    • HeLa and 293 cells; HeLa, HepG2 and 293 cell lysates.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-CREB3 antibody [EPR14215(B)] (ab180119)
    Western blot - Anti-CREB3 antibody [EPR14215(B)] (ab180119)
    Anti-CREB3 antibody [EPR14215(B)] (ab180119) at 1/2000 dilution + HeLa cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 44 kDa
    Observed band size: 40 kDa
    why is the actual band size different from the predicted?

  • Western blot - Anti-CREB3 antibody [EPR14215(B)] (ab180119)
    Western blot - Anti-CREB3 antibody [EPR14215(B)] (ab180119)
    All lanes : Anti-CREB3 antibody [EPR14215(B)] (ab180119) at 1/10000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : 293 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 44 kDa
    Observed band size: 40 kDa why is the actual band size different from the predicted?

  • Immunocytochemistry/ Immunofluorescence - Anti-CREB3 antibody [EPR14215(B)] (ab180119)
    Immunocytochemistry/ Immunofluorescence - Anti-CREB3 antibody [EPR14215(B)] (ab180119)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed 293 cells labeling CREB3 with ab180119 at 1/100 dilution. Counterstained with DAPI (blue).

  • Flow Cytometry - Anti-CREB3 antibody [EPR14215(B)] (ab180119)
    Flow Cytometry - Anti-CREB3 antibody [EPR14215(B)] (ab180119)

    Flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling CREB3 with ab180119 at 1/100 dilution (red) compared to a rabbit IgG control (green), followed by secondary Goat anti rabbit IgG (FITC) at 1/75 dilution.

  • Anti-CREB3 antibody [EPR14215(B)] (ab180119)
    Anti-CREB3 antibody [EPR14215(B)] (ab180119)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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