Anti-PHOS/PDC antibody [EPR21913] (ab221138)
![Anti-PHOS/PDC antibody [EPR21913] (ab221138)](https://www.abcam.com/ps/products/221/ab221138/Images/ab221138-308540-anti-phos-antibody-epr21913-immunohistochemistry.jpg "Anti-PHOS/PDC antibody [EPR21913] (ab221138)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR21913] to PHOS/PDC
- Suitable for: WB, IHC-P, IHC-Fr, IP
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-PHOS/PDC antibody [EPR21913]
See all PHOS/PDC primary antibodies -
Description
Rabbit monoclonal [EPR21913] to PHOS/PDC -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-Fr MouseRatIHC-P MouseRatHumanIP MouseWB MouseRatHuman
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Immunogen
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human eye tissue lysate; Mouse and rat retina tissue lysate. IHC-P: Mouse, rat and human retina tissues. IHC-Fr: Mouse and rat retina tissues. IP: Mouse eyeball tissue lysate.
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General notes
This product was previously labelled as PHOS
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR21913 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Frozen sections) - Anti-PHOS/PDC antibody [EPR21913] (ab221138)
Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized mouse retina tissue labeling PHOS/PDC with ab221138 at 1/100 dilution, followed by AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution. Positive staining on mouse retina is observed. Counterstained with DAPI (Nuclear).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution.
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![Western blot - Anti-PHOS/PDC antibody [EPR21913] (ab221138)](https://www.abcam.com/ps/products/221/ab221138/Images/ab221138-308539-anti-phos-antibody-epr21913-western-blot.jpg)
Western blot - Anti-PHOS/PDC antibody [EPR21913] (ab221138)All lanes : Anti-PHOS/PDC antibody [EPR21913] (ab221138) at 1/1000 dilution
Lane 1 : Human eye tissue lysate
Lane 2 : Mouse retina tissue lysate
Lane 3 : Rat retina tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 28 kDa
Exposure time: 15 secondsBlocking/diluting buffer and concentration: 5% NFDM/TBST.
The molecular mass observed is consistent with the literature (PMID 8816766).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHOS/PDC antibody [EPR21913] (ab221138)](https://www.abcam.com/ps/products/221/ab221138/Images/ab221138-308542-anti-phos-antibody-epr21913-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHOS/PDC antibody [EPR21913] (ab221138)Immunohistochemical analysis of paraffin-embedded mouse retina tissue labeling PHOS/PDC with ab221138 at 1/2000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) ready to use. Positive staining on mouse retina, performed on a Leica Biosystems BOND® RX instrument (PMID: 11287646, PMID: 10617777) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) ready to use.Antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHOS/PDC antibody [EPR21913] (ab221138)](https://www.abcam.com/ps/products/221/ab221138/Images/ab221138-308543-anti-phos-antibody-epr21913-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHOS/PDC antibody [EPR21913] (ab221138)Immunohistochemical analysis of paraffin-embedded rat retina tissue labeling PHOS/PDC with ab221138 at 1/2000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) ready to use. Positive staining on rat retina, performed on a Leica Biosystems BOND® RX instrument (PMID: 11287646, PMID: 10617777) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) ready to use.Antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHOS/PDC antibody [EPR21913] (ab221138)](https://www.abcam.com/ps/products/221/ab221138/Images/ab221138-308544-anti-phos-antibody-epr21913-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHOS/PDC antibody [EPR21913] (ab221138)Immunohistochemical analysis of paraffin-embedded human retina tissue labeling PHOS/PDC with ab221138 at 1/2000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) ready to use. Positive staining on human retina, performed on a Leica Biosystems BOND® RX instrument (PMID: 11287646, PMID: 10617777, PMID: 15842737) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) ready to use.
Antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0).
-
![Immunohistochemistry (Frozen sections) - Anti-PHOS/PDC antibody [EPR21913] (ab221138)](https://www.abcam.com/ps/products/221/ab221138/Images/ab221138-308541-anti-phos-antibody-epr21913-immunohistochemistry.jpg)
Immunohistochemistry (Frozen sections) - Anti-PHOS/PDC antibody [EPR21913] (ab221138)Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized rat retina tissue labeling PHOS/PDC with ab221138 at 1/100 dilution, followed by AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution. Positive staining on mouse retina is observed. Counterstained with DAPI (Nuclear).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution.
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![Immunoprecipitation - Anti-PHOS/PDC antibody [EPR21913] (ab221138)](https://www.abcam.com/ps/products/221/ab221138/Images/ab221138-308545-anti-phos-antibody-epr21913-immunoprecipitation.jpg)
Immunoprecipitation - Anti-PHOS/PDC antibody [EPR21913] (ab221138)PHOS/PDC was immunoprecipitated from 0.35 mg of mouse eyeball tissue lysate with ab221138 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab221138 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/5000 dilution.
Lane 1: Mouse eyeball tissue lysate 10 μg (input).
Lane 2: ab221138 IP in mouse eyeball tissue lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab221138 in mouse eyeball lysate.Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.
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![Anti-PHOS/PDC antibody [EPR21913] (ab221138)](https://www.abcam.com/ps/products/221/ab221138/Images/ab221138-8-benefits-of-recombinant-antibodies.png)
Anti-PHOS/PDC antibody [EPR21913] (ab221138)
