Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PHOS/PDC antibody [EPR21913] - BSA and Azide free (ab234531)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21913] to PHOS/PDC - BSA and Azide free
  • Suitable for: WB, IHC-P, IHC-Fr, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-PHOS/PDC antibody [EPR21913] - BSA and Azide free
    See all PHOS/PDC primary antibodies

  • Description

    Rabbit monoclonal [EPR21913] to PHOS/PDC - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, IHC-Fr, IPmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Mouse retina tissue.

  • General notes

    ab234531 is the carrier-free version of ab221138.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR21913

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHOS/PDC antibody [EPR21913] - BSA and Azide free (ab234531)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHOS/PDC antibody [EPR21913] - BSA and Azide free (ab234531)

    Immunohistochemical analysis of paraffin-embedded human retina tissue labeling PHOS/PDC with ab221138 at 1/2000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) ready to use. Positive staining on human retina, performed on a Leica Biosystems BOND® RX instrument (PMID: 11287646, PMID: 10617777, PMID: 15842737) is observed. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) ready to use.

    Antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab221138).

  • Immunoprecipitation - Anti-PHOS/PDC antibody [EPR21913] - BSA and Azide free (ab234531)
    Immunoprecipitation - Anti-PHOS/PDC antibody [EPR21913] - BSA and Azide free (ab234531)

    PHOS/PDC was immunoprecipitated from 0.35 mg of mouse eyeball tissue lysate with ab221138 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab221138 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/5000 dilution.
    Lane 1: Mouse eyeball tissue lysate 10 μg (input).
    Lane 2: ab221138 IP in mouse eyeball tissue lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab221138 in mouse eyeball lysate.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: 30 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab221138).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHOS/PDC antibody [EPR21913] - BSA and Azide free (ab234531)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHOS/PDC antibody [EPR21913] - BSA and Azide free (ab234531)

    Immunohistochemical analysis of paraffin-embedded rat retina tissue labeling PHOS/PDC with ab221138 at 1/2000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) ready to use. Positive staining on rat retina, performed on a Leica Biosystems BOND® RX instrument (PMID: 11287646, PMID: 10617777) is observed. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) ready to use.

    Antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab221138).

  • Immunohistochemistry (Frozen sections) - Anti-PHOS/PDC antibody [EPR21913] - BSA and Azide free (ab234531)
    Immunohistochemistry (Frozen sections) - Anti-PHOS/PDC antibody [EPR21913] - BSA and Azide free (ab234531)

    Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized rat retina tissue labeling PHOS/PDC with ab221138 at 1/100 dilution, followed by AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution. Positive staining on mouse retina is observed. Counterstained with DAPI (Nuclear).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab221138).

  • Immunohistochemistry (Frozen sections) - Anti-PHOS/PDC antibody [EPR21913] - BSA and Azide free (ab234531)
    Immunohistochemistry (Frozen sections) - Anti-PHOS/PDC antibody [EPR21913] - BSA and Azide free (ab234531)

    Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized mouse retina tissue labeling PHOS/PDC with ab221138 at 1/100 dilution, followed by AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution. Positive staining on mouse retina is observed. Counterstained with DAPI (Nuclear).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab221138).

  • Anti-PHOS/PDC antibody [EPR21913] - BSA and Azide free (ab234531)
    Anti-PHOS/PDC antibody [EPR21913] - BSA and Azide free (ab234531)

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