Anti-PHOS/PDC antibody (ab77523)
Key features and details
- Rabbit polyclonal to PHOS/PDC
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat
- Isotype: IgG
Overview
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Product name
Anti-PHOS/PDC antibody
See all PHOS/PDC primary antibodies -
Description
Rabbit polyclonal to PHOS/PDC -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat
Predicted to work with: Horse, Cow, Cat, Dog, Human
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Immunogen
Synthetic peptide corresponding to Human PHOS/PDC aa 100-200 conjugated to keyhole limpet haemocyanin.
(Peptide available asab88956) -
Positive control
- This antibody gave a positive signal in the following tissue lysates: Mouse Retina; Rat Retina; Rat Eye.
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General notes
This product was previously labelled as PHOS
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab77523 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa). IHC-P Use a concentration of 1 µg/ml. Target
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Function
May participate in the regulation of visual phototransduction or in the integration of photoreceptor metabolism. Inhibits the transcriptional activation activity of the cone-rod homeobox CRX. -
Sequence similarities
Belongs to the phosducin family. -
Post-translational
modificationsLight-induced changes in cyclic nucleotide levels modulate the phosphorylation of this protein by cAMP kinase. -
Cellular localization
Nucleus and Cytoplasm > cytosol. Cell projection > cilium > photoreceptor outer segment. Photoreceptor inner segment. Nucleus. Outer and inner segments of the rod cells. - Information by UniProt
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Database links
- Entrez Gene: 751516 Cat
- Entrez Gene: 287007 Cow
- Entrez Gene: 403624 Dog
- Entrez Gene: 5132 Human
- Entrez Gene: 20028 Mouse
- Entrez Gene: 25343 Rat
- Omim: 171490 Human
- SwissProt: P19632 Cow
see all -
Alternative names
- 33 kDa phototransducing protein antibody
- MEKA protein antibody
- pdc antibody
see all
Images
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Western blot - Anti-PHOS/PDC antibody (ab77523)All lanes : Anti-PHOS/PDC antibody (ab77523) at 1 µg/ml
Lane 1 : Mouse Retina Tissue Lysate
Lane 2 : Rat Retina Tissue Lysate
Lane 3 : Rat Eye Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 28 kDa
Observed band size: 28 kDa
Exposure time: 6 minutes -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHOS/PDC antibody (ab77523)IHC image of ab77523 staining in Mouse Eye formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab77523, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Protocols
Datasheets and documents
References (1)
ab77523 has been referenced in 1 publication.
- Lastowska M et al. Medulloblastoma with transitional features between Group 3 and Group 4 is associated with good prognosis. J Neurooncol 138:231-240 (2018). PubMed: 29427151
Images
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Western blot - Anti-PHOS/PDC antibody (ab77523)All lanes : Anti-PHOS/PDC antibody (ab77523) at 1 µg/ml
Lane 1 : Mouse Retina Tissue Lysate
Lane 2 : Rat Retina Tissue Lysate
Lane 3 : Rat Eye Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 28 kDa
Observed band size: 28 kDa
Exposure time: 6 minutes
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHOS/PDC antibody (ab77523)IHC image of ab77523 staining in Mouse Eye formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab77523, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
