Anti-GIP antibody (ab72167)
Key features and details
- Rabbit polyclonal to GIP
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-GIP antibody
See all GIP primary antibodies -
Description
Rabbit polyclonal to GIP -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human GIP aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab134668) -
Positive control
- This antibody gave a positive signal in Human Pancreas tissue lysate within western blot and human pancreas FFPE tissue sections.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab72167 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species IHC-P HumanWB HumanAll applications ChimpanzeeMacaque monkeyGorillaApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 15 kDa (predicted molecular weight: 17 kDa).IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 15 kDa (predicted molecular weight: 17 kDa).IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.Target
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Function
Potent stimulator of insulin secretion and relatively poor inhibitor of gastric acid secretion. -
Sequence similarities
Belongs to the glucagon family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 2695 Human
- Omim: 137240 Human
- SwissProt: P09681 Human
- Unigene: 1454 Human
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Alternative names
- Gastric Inhibitory Peptide antibody
- Gastric inhibitory polypeptide antibody
- Gastric inhibitory polypeptide precursor antibody
see all
Images
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Western blot - Anti-GIP antibody (ab72167)Anti-GIP antibody (ab72167) at 1 µg/ml + Human pancreas tissue lysate - total protein (ab29816) at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 17 kDa
Observed band size: 15 kDa why is the actual band size different from the predicted?
Additional bands at: 95 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 12 minutes
The band observed at 15 kDa could potentially be a cleaved form of GIP due to the presence of a 21 amino acid signal peptide. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GIP antibody (ab72167)IHC image of ab72167 staining in human pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab72167, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Protocols
Datasheets and documents
References (0)
ab72167 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-GIP antibody (ab72167)Anti-GIP antibody (ab72167) at 1 µg/ml + Human pancreas tissue lysate - total protein (ab29816) at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 17 kDa
Observed band size: 15 kDa why is the actual band size different from the predicted?
Additional bands at: 95 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 12 minutes
The band observed at 15 kDa could potentially be a cleaved form of GIP due to the presence of a 21 amino acid signal peptide. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GIP antibody (ab72167)IHC image of ab72167 staining in human pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab72167, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
