Anti-GIP antibody (ab83177)
Key features and details
- Rabbit polyclonal to GIP
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-GIP antibody
See all GIP primary antibodies -
Description
Rabbit polyclonal to GIP -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human GIP aa 50-150 conjugated to keyhole limpet haemocyanin.
(Peptide available asab94377) -
Positive control
- Recombinant Human GIP protein (ab116416) can be used as a positive control in WB. This antibody gave a positive signal in the following lysates: HEK293 whole cell; A498 whole cell; MDA-MB-361 whole cell; JEG-3 whole cell; Human Placenta Tissue
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General notes
Previously labelled as CTRP1.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab83177 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanApplication Abreviews Notes WB Notes Application notesWB: Use at a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 32 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.Target
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Sequence similarities
Contains 1 C1q domain.
Contains 1 collagen-like domain. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 114897 Human
- GenBank: NP_940995 Human
- Omim: 610365 Human
- SwissProt: Q9BXJ1 Human
- Unigene: 201398 Human
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Alternative names
- C1q and tumor necrosis factor related protein 1 antibody
- C1QT1_HUMAN antibody
- C1QTNF1 antibody
see all
Images
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Western blot - Anti-GIP antibody (ab83177)All lanes : Anti-GIP antibody (ab83177) at 1 µg/ml
Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 2 : A498 (Human Kidney Carcinoma) Whole Cell Lysate
Lane 3 : MDA-MB-361 (Human breast adenocarcinoma cell line) Whole Cell Lysate Whole Cell Lysate
Lane 4 : JEG-3 (Human placental choriocarcinoma cell line) Whole Cell Lysate
Lane 5 : Human placenta tissue lysate - total protein (ab29745)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 32 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
Additional bands at: 21 kDa. We are unsure as to the identity of these extra bands.GIP contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
Datasheets and documents
References (0)
ab83177 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-GIP antibody (ab83177)All lanes : Anti-GIP antibody (ab83177) at 1 µg/ml
Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 2 : A498 (Human Kidney Carcinoma) Whole Cell Lysate
Lane 3 : MDA-MB-361 (Human breast adenocarcinoma cell line) Whole Cell Lysate Whole Cell Lysate
Lane 4 : JEG-3 (Human placental choriocarcinoma cell line) Whole Cell Lysate
Lane 5 : Human placenta tissue lysate - total protein (ab29745)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 32 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
Additional bands at: 21 kDa. We are unsure as to the identity of these extra bands.GIP contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
