Orexin A (bovine, human, mouse, rat), Neuropeptide involved in feeding and sleep (ab120212)
Key features and details
- Neuropeptide involved in feeding and sleep
- CAS Number: 205640-90-0
- Purity: > 90%
- Soluble in water to 1mg/ml
- Form / State: Solid
- Source: Synthetic
Overview
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Product name
Orexin A (bovine, human, mouse, rat), Neuropeptide involved in feeding and sleep -
Description
Neuropeptide involved in feeding and sleep -
Biological description
Hypothalamic neuropeptide involved in the regulation of feeding, sleep and wakefulness.
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Purity
> 90% -
CAS Number
205640-90-0 -
Chemical structure
Properties
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Molecular weight
3561.12 -
Molecular formula
C152H243N47O44S4 -
Sequence
XPLPDCCRQKTCSCRLYELLHGAGNHAAGILTL (Modifications: C-terminal amide; X-1 = Glp; Disulfide bonds: 6-12, 7-14) -
PubChem identifier
56842143 -
Storage instructions
Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
Solubility overview
Soluble in water to 1mg/ml -
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one week. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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Source
Synthetic
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Research areas
Images
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Functional Studies - Orexin A (bovine, human, mouse, rat), Neuropeptide involved in feeding and sleep (ab120212)ab6211 staining tyrosine hydroxylase in PC-12 cells treated with orexin A (ab120212), by ICC/IF. Decrease in expression of tyrosine hydroxylase correlates with increased concentration of orexin A, as described in literature.
The cells were incubated at 37°C for 1h in media containing different concentrations of ab120212 (orexin A) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab6211 (1/1000 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.