Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PDGFR beta (phospho Y751) antibody [EPR21744] - BSA and Azide free (ab237781)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21744] to PDGFR beta (phospho Y751) - BSA and Azide free
  • Suitable for: WB, Dot blot
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-PDGFR beta (phospho Y751) antibody [EPR21744] - BSA and Azide free
    See all PDGFR beta primary antibodies

  • Description

    Rabbit monoclonal [EPR21744] to PDGFR beta (phospho Y751) - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Dot
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: IMR-90 (treated with 50 ng/ml PDGF-BB for 5 minutes), NIH/3T3 (treated with 50 ng/ml PDGF-BB for 5 minutes) and C6 (treated with 50 ng/ml PDGF-BB for 5 minutes) whole cell lysates.

  • General notes

    Ab237781 is the carrier-free version of ab218534. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab237781 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Dot Blot - Anti-PDGFR beta (phospho Y751) antibody [EPR21744] - BSA and Azide free (ab237781)
    Dot Blot - Anti-PDGFR beta (phospho Y751) antibody [EPR21744] - BSA and Azide free (ab237781)

    Dot blot analysis of PDGFR beta (phospho Y751) labeled with ab218534 at 1/1000 dilution.

    Lane 1: Human PDGFR beta (phospho Y751) peptide.
    Lane 2: Human PDGFR beta non-phospho peptide.

    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution was used as secondary antibody.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab218534).

  • Western blot - Anti-PDGFR beta (phospho Y751) antibody [EPR21744] - BSA and Azide free (ab237781)
    Western blot - Anti-PDGFR beta (phospho Y751) antibody [EPR21744] - BSA and Azide free (ab237781)
    All lanes : Anti-PDGFR beta (phospho Y751) antibody [EPR21744] (ab218534) at 1/1000 dilution

    Lane 1 : Untreated IMR-90 (human lung fibroblast cell line) whole cell lysate
    Lane 2 : IMR-90 (treated with 50 ng/ml PDGF-BB for 5 minutes) whole cell lysate
    Lane 3 : Untreated NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
    Lane 4 : NIH/3T3 (treated with 50 ng/ml PDGF-BB for 5 minutes) whole cell lysate
    Lane 5 : Untreated C6 (rat glial tumor glial cell line) whole cell lysate
    Lane 6 : C6 (treated with 50 ng/ml PDGF-BB for 5 minutes) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 124 kDa
    Observed band size: 124,190 kDa
    why is the actual band size different from the predicted?



    Exposure times : Lanes 1-4: 26 seconds, Lanes 5 & 6: 3 minutes.

    Blocking and dilution buffer: 1% BSA/TBST.

    Expression of PDGFR beta (phospho Y751) is induced by PDGF-BB. The antibody recognises both the glycosylated and unglycosylated protein (PMID: 9307305).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab218534).

  • Anti-PDGFR beta (phospho Y751) antibody [EPR21744] - BSA and Azide free (ab237781)
    Anti-PDGFR beta (phospho Y751) antibody [EPR21744] - BSA and Azide free (ab237781)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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