Anti-PAX7 antibody (ab187339)
Key features and details
- Rabbit polyclonal to PAX7
- Suitable for: ICC/IF, WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PAX7 antibody
See all PAX7 primary antibodies -
Description
Rabbit polyclonal to PAX7 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human PAX7 (internal sequence). The exact sequence is proprietary. (Purified from E. coli).
Database link: P23759 -
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 69% PBS, 30% Glycerol, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PAX7 antibody (ab187339)All lanes : Anti-PAX7 antibody (ab187339) at 1/1000 dilution
Lane 1 : HeLa lysate transfected with empty vector control
Lane 2 : HeLa lysate overexpressing PAX7
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 56 kDa
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Western blot - Anti-PAX7 antibody (ab187339)All lanes : Anti-PAX7 antibody (ab187339) at 1/1000 dilution
Lane 1 : HeLa whole cell lysate
Lane 2 : MCF7 whole cell lysate
Lane 3 : HSkMSC whole cell lysate
Lane 4 : SJCRH30 whole cell lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 56 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-PAX7 antibody (ab187339)Immunofluorescent analysis of PAX7 in MCF7 (left panel) and HeLa (right panel) cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were probed with a ab187339 at 1/100 dilution for at least 1 hour at room temperature, washed with PBS, and incubated with a DyLight 488-conjugated goat anti-rabbit IgG secondary antibody at 1/400 dilution for 30 minutes at room temperature. F-Actin (red) was stained with DyLight-554 Phalloidin and nuclei (blue) were stained with Hoechst 33342 dye.
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Immunoprecipitation - Anti-PAX7 antibody (ab187339)Immunoprecipitation of PAX7 was performed using HeLa whole cell lysate. Antigen-antibody complexes were formed by incubating 500µg of lysate with 3µg of ab187339 overnight on a rocking platform at 4°C. The immune complexes were captured on 50ul Protein A/G Agarose, washed extensively, and eluted. Samples was resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% BSA/TBS-0.1%Tween-20 for 1 hour. The membrane was probed with ab187339 at 1/1000 dilution overnight rotating at 4°C.
