Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PAX1 antibody [5A2] - BSA and Azide free (ab255841)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rat monoclonal [5A2] to PAX1 - BSA and Azide free
  • Suitable for: IHC-Fr, IHC-P
  • Reacts with: Mouse

Overview

  • Product name

    Anti-PAX1 antibody [5A2] - BSA and Azide free
    See all PAX1 primary antibodies

  • Description

    Rat monoclonal [5A2] to PAX1 - BSA and Azide free

  • Host species

    Rat

  • Tested Applications & Species

    Application Species
    IHC-Fr
    Mouse
    IHC-P
    Mouse
    See all applications and species data

  • Immunogen

    Recombinant fragment within Mouse PAX1. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • IHC-P: Mouse embroy day 14.5 tissue. IHC-Fr: Mouse E14.5 embryo tissue.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    ab255841 is the carrier-free version of ab252847. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Immunohistochemistry (Frozen sections) - Anti-PAX1 antibody [5A2] - BSA and Azide free (ab255841)
    Immunohistochemistry (Frozen sections) - Anti-PAX1 antibody [5A2] - BSA and Azide free (ab255841)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse E14.5 embryo tissue labeling PAX1 with ab252847 at 28.76 µg/mL followed by ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) at dilution (Green). Positive staining on mouse embryo is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488)at dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252847).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX1 antibody [5A2] - BSA and Azide free (ab255841)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX1 antibody [5A2] - BSA and Azide free (ab255841)

    Immunohistochemical analysis of paraffin-embedded mouse embroy day 14.5 tissue labeling PAX1 with ab252847 at 1.438 µg/mL followed by a ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Nuclear staining on the intervertebral disk in mouse embroy day 14.5. The section was incubated with ab252847 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252847).

  • Anti-PAX1 antibody [5A2] - BSA and Azide free (ab255841)
    Anti-PAX1 antibody [5A2] - BSA and Azide free (ab255841)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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