Anti-PAX3 antibody [C2] (ab69856)
Key features and details
- Mouse monoclonal [C2] to PAX3
- Suitable for: IHC-P, Flow Cyt, WB
- Reacts with: Human
- Isotype: IgG2a
Overview
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Product name
Anti-PAX3 antibody [C2]
See all PAX3 primary antibodies -
Description
Mouse monoclonal [C2] to PAX3 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIHC-P HumanWB Human -
Immunogen
Synthetic peptide (quail) from the C-terminus
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Positive control
- 624 Mel nuclear lysate; Human skeletal muscle and Human skin tissue
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol, PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
C2 -
Isotype
IgG2a -
Research areas
Images
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ab109691, at 10 µg/ml, staining PAX3 in formalin-fixed, paraffin-embedded Human Skeletal muscle tissue by Immunohistochemistry.
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All lanes : Anti-PAX3 antibody [C2] (ab69856) at 1 µg/ml
Lane 1 : Marker
Lane 2 : 624 Mel nuclear lysate
Developed using the ECL technique.
Predicted band size: 53 kDa
Observed band size: 53 kDa
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ab109691, at 10 µg/ml, staining PAX3 in formalin-fixed, paraffin-embedded Human Skin tissue by Immunohistochemistry.
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Overlay histogram showing K562 cells stained with ab69856 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab69856, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in K562 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.