Anti-PAX3 antibody (ab15717)
Key features and details
- Goat polyclonal to PAX3
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PAX3 antibody
See all PAX3 primary antibodies -
Description
Goat polyclonal to PAX3 -
Host species
Goat -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat, Chicken, Dog
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Immunogen
Synthetic peptide: TTLAGAVPRMM, corresponding to N terminal amino acids 2-12 of PAX3.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX3 antibody (ab15717) Image courtesy of an anonymous Abreview.ab15717 staining PAX3 in murine striated muscle tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
Tissue was fixed in formaldehyde and a heat mediated antigen retrieval step was performed using citrated buffer pH 6. Samples were then blocked with 1% BSA for 30 minutes at 25°C and then incubated with ab15717 at a 1/100 dilution for 18 hours at 25°C. The secondary used was an undiluted HRP conjugated goat polyclonal. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX3 antibody (ab15717)ab15717 (3µg/ml) staining of paraffin embedded Human Oesophagus. The tissue sections subjected to antigen retrieval by microwave in Tris/EDTA buffer pH 9.0. The HRP-staining procedure was used for detection.
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Western blot - Anti-PAX3 antibody (ab15717)Anti-PAX3 antibody (ab15717) at 0.3 µg/ml + Human duodenum lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 53 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
