Anti-PAX1 antibody [5A2] (ab252847)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rat monoclonal [5A2] to PAX1
- Suitable for: IHC-Fr, IHC-P
- Reacts with: Mouse
Overview
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Product name
Anti-PAX1 antibody [5A2]
See all PAX1 primary antibodies -
Description
Rat monoclonal [5A2] to PAX1 -
Host species
Rat -
Tested Applications & Species
See all applications and species dataApplication Species IHC-Fr MouseIHC-P Mouse -
Immunogen
Full length protein (GST-tag) corresponding to Mouse PAX1.
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Positive control
- IHC-P: Mouse embroy day 14.5 tissue. IHC-Fr: Mouse E14.5 embryo tissue.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Ion Exchange Chromatography -
Clonality
Monoclonal -
Clone number
5A2 -
Isotype
IgG2a -
Research areas
Images
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Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse E14.5 embryo tissue labeling PAX1 with ab252847 at 128.76 µg/mL followed by ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) at dilution (Green). Positive staining on mouse embryo is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488)at dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
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Immunohistochemical analysis of paraffin-embedded mouse embroy day 14.5 tissue labeling PAX1 with ab252847 at 1.438 μg/mL followed by a ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Nuclear staining on the intervertebral disk in mouse embroy day 14.5. The section was incubated with ab252847 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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