Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PAX1 antibody [5A2] (ab252847)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rat monoclonal [5A2] to PAX1
  • Suitable for: IHC-Fr, IHC-P
  • Reacts with: Mouse

Overview

  • Product name

    Anti-PAX1 antibody [5A2]
    See all PAX1 primary antibodies

  • Description

    Rat monoclonal [5A2] to PAX1

  • Host species

    Rat

  • Tested Applications & Species

    Application Species
    IHC-Fr
    Mouse
    IHC-P
    Mouse
    See all applications and species data

  • Immunogen

    Full length protein (GST-tag) corresponding to Mouse PAX1.

  • Positive control

    • IHC-P: Mouse embroy day 14.5 tissue. IHC-Fr: Mouse E14.5 embryo tissue.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunohistochemistry (Frozen sections) - Anti-PAX1 antibody [5A2] (ab252847)
    Immunohistochemistry (Frozen sections) - Anti-PAX1 antibody [5A2] (ab252847)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse E14.5 embryo tissue labeling PAX1 with ab252847 at 128.76 µg/mL followed by ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) at dilution (Green). Positive staining on mouse embryo is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488)at dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX1 antibody [5A2] (ab252847)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX1 antibody [5A2] (ab252847)

    Immunohistochemical analysis of paraffin-embedded mouse embroy day 14.5 tissue labeling PAX1 with ab252847 at 1.438 μg/mL followed by a ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Nuclear staining on the intervertebral disk in mouse embroy day 14.5. The section was incubated with ab252847 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Anti-PAX1 antibody [5A2] (ab252847)
    Anti-PAX1 antibody [5A2] (ab252847)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Clear all

Recently viewed products

© 2021 Astana Biomed Group LLP. All rights reserved.